Identification of protein scaffolds for enzyme design using scaffold selection.
Identification of protein scaffolds for enzyme design using scaffold selection.
复制标题
DOI：
10.1007/978-1-4939-1486-9_9
复制
发表时间：
2014
影响因子：
--
通讯作者：
A. Stiel;K. Feldmeier;B. Höcker
中科院分区：
文献类型：
--
作者：
A. Stiel;K. Feldmeier;B. Höcker
确定合适的蛋白质结构，可以作为引入催化残基的支架，对于新酶的设计至关重要。在这里，我们描述了如何使用自动化和快速的支架搜索程序ScaffoldSelection来找到最佳的起点，即最有可能耐受引入并促进特定催化基序的正确形成的蛋白质结构。
The identification of suitable protein structures that can serve as scaffolds for the introduction of catalytic residues is crucial for the design of new enzymes. Here we describe how the automated and rapid scaffold search program ScaffoldSelection can be used to find the best starting points, namely protein structures that are most likely to tolerate the introduction and promote the proper formation of a specific catalytic motif.