The effect of chronic and acute low pH on the intracellular DMSP production and epithelial cell morphology of red coralline algae
The effect of chronic and acute low pH on the intracellular DMSP production and epithelial cell morphology of red coralline algae
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DOI：
10.1080/17451000.2012.676189
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发表时间：
2012-01-01
影响因子：
1.1
通讯作者：
Kamenos, Nicholas A.
中科院分区：
文献类型：
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作者：
Burdett, Heidi L.;Aloisio, Elena;Kamenos, Nicholas A.
海藻释放的二甲基磺基丙酸盐（DMSP）对全球硫循环产生重大影响，并可能通过形成二甲基硫（DMS）影响当地气候。然而，全球变化对藻类生产DMSP/DMS（DMS（P））的影响尚未得到充分了解。本研究探讨了低pH值对自由生活的红珊瑚石羊膜（Lithothamnion glaciale）的DMS（P）产生和上皮细胞形态的影响。在80天实验中使用三种pH处理：（1）当前pH值（8.18，对照），（2）代表2100年海洋酸化（OA）情景的低慢性pH值（7.70）和（3）低、急性pH值（7.75，3天后飙升至6.47），代表可能与碳捕获和储存基础设施泄漏有关的急性可变条件，在CO2喷口或上升流区域。在低的、稳定的pH条件下，DMS（P）的产生没有显著增加，这表明红珊瑚藻可能对OA具有一定的适应性。然而，细胞内和水柱DMS（P）的浓度显着高于对照组时，pH值急剧增加。在两种低pH处理中，藻骨架的细胞壁之间观察到裂纹。有人提出，这种结构变化可能会导致膜损伤，使DMS（P）从细胞泄漏到水柱中，随后影响到DMS（P）在珊瑚藻栖息地的循环。
The release of dimethylsulphoniopropionate (DMSP) by marine algae has major impacts on the global sulphur cycle and may influence local climate through the formation of dimethylsulphide (DMS). However, the effect of global change on DMSP/DMS (DMS(P)) production by algae is not well understood. This study examined the effect of low pH on DMS(P) production and epithelial cell morphology of the free-living red coralline alga Lithothamnion glaciale. Three pH treatments were used in the 80-day experiment: (1) current pH level (8.18, control), (2) low, chronic pH representing a 2100 ocean acidification (OA) scenario (7.70) and (3) low, acute pH (7.75, with a 3-day spike to 6.47), representing acute variable conditions that might be associated with leaks from carbon capture and storage infrastructure, at CO2 vent sites or in areas of upwelling. DMS(P) production was not significantly enhanced under low, stable pH conditions, indicating that red coralline algae may have some resilience to OA. However, intracellular and water column DMS(P) concentrations were significantly higher than the control when pH was acutely spiked. Cracks were observed between the cell walls of the algal skeleton in both low pH treatments. It is proposed that this structural change may cause membrane damage that allows DMS(P) to leak from the cells into the water column, with subsequent implications for the cycling of DMS(P) in coralline algae habitats.