Expression of protocadherin 18 in the CNS and pharyngeal arches of zebrafish embryos
Expression of protocadherin 18 in the CNS and pharyngeal arches of zebrafish embryos
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DOI：
10.1387/ijdb.072424fk
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发表时间：
2008-01-01
影响因子：
0.7
通讯作者：
Yorifuji, Hiroshi
中科院分区：
文献类型：
--
作者：
Kubota, Fumitaka;Murakami, Tohru;Yorifuji, Hiroshi
在这里，我们报告的非成簇原钙粘蛋白（pcdh），pcdh 18在斑马鱼胚胎中的分子克隆和表达分析的结果。预测的斑马鱼pcdh 18蛋白显示65-66%的同一性和78-79%的同源性，其哺乳动物和非洲爪蟾对应。它在其胞质结构域中具有Disabled-1结合基序，这是pcdh 18的特征。斑马鱼胚胎在早期原肠胚阶段，受精后6 h（hpf），在其动物帽中表达pcdh 18，但在胚环或盾中不表达。pcdh 18从12 hpf开始在神经管和中枢神经系统（CNS）中表达。12-18 hpf胚胎的侧神经管和脊髓中的一些细胞群体表达pcdh 18，但这些细胞中的表达在24 hpf时消失。在24-56 hpf的胚胎后脑表达pcdh 18细胞紧密相邻的吻和尾菱形边界在一个线程样的模式运行在背腹方向。pcdh 18阳性细胞在24 hpf和36 hpf时分别位于后脑腹侧和背侧。pcdh 18在端脑、间脑、顶盖、上菱形唇、视网膜和耳泡中也有表达。在CNS中的表达在孵化前显著降低。咽弓原基、弓、颌和鳃表达pcdh 18，该分子在胚胎晚期的一些内胚层细胞中也有表达。
Here, we report the results of molecular cloning and expression analyses of a non-clustered protocadherin (pcdh), pcdh18 in zebrafish embryos. The predicted zebrafish pcdh18 protein shows 65-66% identity and 78-79% homology with its mammalian and Xenopus counterparts. It has a Disabled-1 binding motif in its cytoplasmic domain, which is characteristic of pcdh18. Zebrafish embryos expressed pcdh18 by the early gastrula stage, 6 h post-fertilization (hpf), in their animal cap but not in the germ ring or the shield. pcdhl8 was expressed in the neural tube and the central nervous system (CNS) from 12 hpf. Some populations of cells in the lateral neural tube and spinal cord of 12-18 hpf embryos expressed pcdh18, but expression in these cells disappeared by 24 hpf. The hindbrain of embryos at 24-56 hpf expressed pcdh18 in cells closely adjacent to the rostral and caudal rhombomeric boundaries in a thread-like pattern running in the dorsoventral direction. The pcdh18-positive cells were localized in the ventral part of the hindbrain at 24 hpf and in the dorsal part from 36 hpf. pcdh18 was also expressed in the telencephalon, diencephalon, tectum, upper rhombic lip, retina and otic vesicle. Expression in the CNS decreased markedly before hatching. Pharyngeal arch primordia, arches, jaws and gills expressed pcdh18, and the molecule was also expressed in some endodermal cells in late embryos.