Chitosan enhances platelet adhesion and aggregation
Chitosan enhances platelet adhesion and aggregation
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DOI：
10.1016/s0006-291x(03)00173-6
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发表时间：
2003-03-14
影响因子：
3.1
通讯作者：
Yeh, JH
中科院分区：
文献类型：
--
作者：
Chou, TC;Fu, E;Yeh, JH
本研究以兔血小板悬液为模型，考察了壳聚糖（MW = 50，000）对血小板活性的增强作用，并探讨了其可能的作用机制。我们的研究结果表明，在初始（5分钟）和长期（30分钟）接触的血小板与壳聚糖，血小板粘附壳聚糖涂层的微量滴定板相比，溶剂对照剂量依赖性增加。壳聚糖也呈剂量依赖性地增加Fura-2-AM负载血小板的聚集率和胞内游离Ca ~（2+）升高。此外，在FITC标记的抗CD 41/CD 61存在下，壳聚糖显著增强血小板糖蛋白IIb/IIIa复合物的表达，通过流式细胞仪测定。结论：壳聚糖是一种有效的血小板粘附和聚集诱导剂，其作用机制可能与其增加血小板膜表面[Ca ~（2+）]（i）动员和GPIIb/IIIa复合物表达有关。(C)2003 Elsevier Science（美国）。All rights reserved.
In this study, chitosan (MW = 50,000) was tested for its enhancing platelet activity in rabbit platelet suspensions and the possible mechanisms involved were further investigated. Our results showed that after initial (5 min) and long-term (30 min) contact of platelets with chitosan, the platelet adhesion to chitosan-coated microtiter plates was dose-dependently increased compared to that of solvent control. Similarly, chitosan also dose-dependently increased the platelet aggregation and the intracellular free Ca2+ rise of Fura-2-AM loaded platelets. Additionally, in the presence of FITC-labeled anti-CD41/CD61, chitosan significantly enhanced the expression of platelet glycoprotein IIb/IIIa complex assayed by a flow cytometer. It is concluded that chitosan is an effective inducer for platelet adhesion and aggregation and the mechanisms of action of chitosan may be associated, at least partly, with the increasing [Ca2+](i) mobilization and enhancing expression of GPIIb/IIIa complex on platelet membrane surfaces. (C) 2003 Elsevier Science (USA). All rights reserved.