Quadruplex-to-duplex transition of G-rich oligonucleotides probed by cationic water-soluble conjugated polyelectrolytes.
Quadruplex-to-duplex transition of G-rich oligonucleotides probed by cationic water-soluble conjugated polyelectrolytes.
复制标题
DOI：
10.1021/ja058075w
复制
发表时间：
2006-05
影响因子：
15
通讯作者：
F. He;Yanli Tang;Minghui Yu;F. Feng;Lingling An;Huan Sun;Shu Wang;Yuliang Li;Daoben Zhu
中科院分区：
文献类型：
--
作者：
F. He;Yanli Tang;Minghui Yu;F. Feng;Lingling An;Huan Sun;Shu Wang;Yuliang Li;Daoben Zhu
G 四重体 DNA 在其互补链存在的情况下转化为双链体形式。这种构象变化可以通过基于共轭聚电解质信号放大和嵌入染料与双链 DNA (dsDNA) 的特异性相互作用的均相测定方法实时检测。探针溶液含有阳离子共轭聚合物 (CCP)、5' 末端用荧光素标记的 G-四链体 (G-quadruplex-Fl) 和溴化乙锭 (EB)。互补靶标的添加导致从 G-四链体到双链体 (dsDNA-F1) 的转变以及双链体结构内的 EB 嵌入。 CCP的激发导致能量从CCP转移到dsDNA-F1 (FRET-1)，然后能量从dsDNA-F1转移到EB (FRET-2)。增加错配碱基的数量会阻碍双链 DNA 的形成，而双链 DNA 的形成是在测定中检测到的。
G-quartet DNA converts to duplex form in the presence of its complementary strand. This conformational change can be detected in real time by a homogeneous assay method based on the signal amplification of conjugated polyelectrolytes and the specific interaction of intercalating dyes with double-stranded DNA (dsDNA). The probe solution contains a cationic, conjugated polymer (CCP), G-quadruplex labeled with a fluorescein at the 5'-terminus (G-quadruplex-Fl), and ethidium bromide (EB). The addition of a complementary target results in the transition from G-quadruplex to duplex (dsDNA-Fl) and EB intercalation within the duplex structure. Excitation of the CCP leads to energy transfer from CCP to dsDNA-Fl (FRET-1) and then energy transfer from dsDNA-Fl to EB (FRET-2). Increasing the number of mismatched bases discourages dsDNA formation, which is detected in the assay.