An atherogenic lipid profile with low serum paraoxonase-1 activity during nematode infection in rats
An atherogenic lipid profile with low serum paraoxonase-1 activity during nematode infection in rats
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DOI：
10.1111/j.1365-2362.2010.02352.x
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发表时间：
2010-11-01
影响因子：
5.5
通讯作者：
Horii, Yoichiro
中科院分区：
文献类型：
--
作者：
Farid, Ayman Samir;Mido, Shogo;Horii, Yoichiro
背景：炎症和氧化应激与心血管疾病和潜在的动脉粥样硬化有关。已知高密度脂蛋白（HDL）相关的对氧磷酶-1 （PON1）酶参与保护血脂免受这种氧化。然而，在线虫感染模型中脂质谱的干扰尚未被研究。因此，我们旨在探讨巴西尼波圆线虫感染雄性Wistar大鼠（人类胃肠道线虫感染模型）对肝脏PON1合成和脂质参数水平的影响。材料与方法巴西芽孢杆菌感染大鼠分别饲喂标准和高脂饲料。分别于感染后第0、2、4、7和14天测定血清对氧磷酶和芳酰酯酶活性。在标准饮食组中评估肝脏pon和促炎细胞因子mRNA表达水平，在高脂肪饮食组中测量脂质谱紊乱以及硫代巴比妥酸活性物质（TBARS）和氧化低密度脂蛋白（Ox-LDL）水平。结果我们发现，饲喂标准饮食的巴西乳杆菌感染大鼠血清PON1活性显著降低，肝脏PON1 mRNA表达下调，肝脏IL-1 β、il - β受体(R)、tnf - α和TNFR1 mRNA表达上调，这与肝脏库普弗细胞和中性粒细胞的募集有关。在高脂肪饮食存在的情况下，巴西巴西螺感染增加血清甘油三酯、总胆固醇、LDL/VLDL、TBARS和Ox-LDL，并降低血清HDL，同时最大限度地降低血清PON1。结论巴西芽孢杆菌感染可引起动脉粥样硬化性血脂变化，降低血清PON1活性。
P>BackgroundInflammation and oxidative stress are associated with cardiovascular diseases and underlying atherosclerosis. The high density lipoprotein (HDL)-associated paraoxonase-1 (PON1) enzyme is known to be involved in the protection of serum lipids from such oxidation. Nonetheless, the disturbances of lipid profile during nematode-infected model have not yet been studied. Therefore, we aimed to explore the effects of Nippostrongylus brasiliensis infection in male Wistar rats, a model of human gastrointestinal nematode infections, on hepatic PON1 synthesis and the levels of lipid parameters.Materials and methodsNippostrongylus brasiliensis-infected rats fed standard and high-fat diets. Serum paraoxonase and arylesterase activities were measured on day 0, 2, 4, 7, and 14 post-infection (PI). Hepatic PONs and pro-inflammatory cytokines mRNA expression levels were evaluated in a standard diet-fed groups, and the disturbances in lipid profile as well as the levels of thiobarbituric acid reactive species (TBARS) and oxidized-LDL (Ox-LDL) were measured in high-fat diet-fed groups.ResultsWe found that N. brasiliensis-infected rats fed the standard diet show a significant reduction in serum PON1 activity and down-regulation of hepatic PON1 mRNA expression as well as up-regulation of hepatic IL-1 beta, IL-beta receptor (R), TNF-alpha, and TNFR1 mRNA expressions in association with hepatic recruitments of Kupffer cells and neutrohils. In the presence of the high-fat diet, N. brasiliensis infection increases serum triglycerides, total cholesterol, LDL/VLDL, TBARS and Ox-LDL as well as decreases serum HDL coinciding with a maximum serum PON1 reduction.ConclusionsNippostrongylus brasiliensis infection can induce atherogenic lipid profile and reduce serum PON1 activity.