INVOLVEMENT OF DNA GYRASE IN REPLICATION AND TRANSCRIPTION OF BACTERIOPHAGE-T7 DNA
INVOLVEMENT OF DNA GYRASE IN REPLICATION AND TRANSCRIPTION OF BACTERIOPHAGE-T7 DNA
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DOI：
10.1128/jvi.29.2.529-535.1979
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发表时间：
1979-01-01
影响因子：
5.4
通讯作者：
HINKLE, DC
中科院分区：
文献类型：
--
作者：
DEWYNGAERT, MA;HINKLE, DC
噬菌体T7的生长受到抗生素香豆霉素A1的抑制，香豆霉素A1是大肠杆菌DNA促旋酶的抑制剂。由于噬菌体的生长对含有香豆霉素抗性DNA促旋酶的菌株中的抗生素不敏感，因此该酶似乎是噬菌体生长所需的。我们研究了香豆霉素对T7感染过程中DNA、RNA和蛋白质合成动力学的影响。DNA合成被抗生素完全抑制。此外，香豆霉素显著抑制晚期基因的转录，但不抑制早期基因的转录。因此，E.大肠杆菌DNA旋转酶在T7 DNA的转录和复制过程中起重要作用。
Growth of bacteriophage T7 is inhibited by the antibiotic coumermycin A1, an inhibitor of the Escherichia coli DNA gyrase. Since growth of the phage is insensitive to the antibiotic in strains containing a coumermycin-resistant DNA gyrase, this enzyme appears to be required for phage growth. We have investigated the effect of coumermycin on the kinetics of DNA, RNA, and protein synthesis during T7 infection. DNA synthesis is completely inhibited by the antibiotic. In addition, coumermycin significantly inhibits transcription of late but not early genes. Thus, E. coli DNA gyrase may play an important role in transcription as well as in replication of T7 DNA.