Drosophila nucleoporin Nup154 controls cell viability, proliferation and nuclear accumulation of Mad transcription factor
Drosophila nucleoporin Nup154 controls cell viability, proliferation and nuclear accumulation of Mad transcription factor
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DOI：
10.1016/j.tice.2011.05.001
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发表时间：
2011-08-01
期刊：
影响因子：
2.6
通讯作者：
Callaini, Giuliano
中科院分区：
文献类型：
--
作者：
Colozza, Gabriele;Montembault, Emilie;Callaini, Giuliano
核孔复合物（Nuclear Pore Complexes，NPC）参与调节核质运输。果蝇Nup 154编码NPC的核孔蛋白组分，其在高增殖组织如生殖细胞中表达。该基因中的亚型突变导致雄性和雌性不育，并减少成蝇的细胞增殖。在这里，我们提出的证据表明，在Nup 154突变体的精原细胞的数量减少，引起细胞死亡增加和细胞增殖减少。此外，我们还发现，RNAi介导的培养细胞中Nup 154的耗竭阻止了针对Dpp（Mad）的转录因子Mothers的核积累，这表明Nup 154在TGF-β信号转导中可能具有调节作用。这些结果在体内突变体睾丸上得到证实，我们在其中观察到co-Smad Medea的核积累中的类似缺陷。(C)2011爱思唯尔有限公司版权所有。
Nuclear Pore Complexes (NPCs) are involved in the regulation of nucleo-cytoplasmic trafficking. Drosophila Nup154 encodes a nucleoporin component of the NPC that is expressed in high proliferating tissues such as germ cells. Hypomorphic mutations in this gene cause male and female sterility and reduction of cell proliferation in the adult fly. Here, we present evidences of a decrease in the number of spermatogonial cells in Nup154 mutants, caused both by increased cell death and reduced cell proliferation. Furthermore, we also found that RNAi-mediated depletion of Nup154 in cultured cells prevented nuclear accumulation of the transcription factor Mothers against Dpp (Mad), suggesting a possible regulatory role for Nup154 in TGF-beta signal transduction. These results were confirmed in vivo on mutant testes where we observed a similar defect in the nuclear accumulation of the co-Smad Medea. (C) 2011 Elsevier Ltd. All rights reserved.