Origination of asexual plantlets in three species of Crassulaceae
Origination of asexual plantlets in three species of Crassulaceae
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三种景天科植物无性幼苗的起源
DOI：
10.1007/s00709-014-0704-2
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发表时间：
2015-03-01
期刊：
影响因子：
2.9
通讯作者：
Zhu, Jian
中科院分区：
文献类型：
--
作者：
Guo, Jiansheng;Liu, Hailiang;Zhu, Jian
在植物无性繁殖过程中，来自不同器官的细胞可以重新编程以产生新的个体，这一过程需要细胞周期再激活与其他细胞形态特征的获得相协调。然而，影响无性繁殖多样性的因素尚未确定。本文报道了景天科植物大叶栎、巴拉圭风车瓣和海桐的成苗过程，并分析了起始细胞对这三种植物无性繁殖的影响。此外，还通过qRT-PCR分析了WUSCHEL（WUS）和Cup-shape Cotyledon 1（CUC 1）在这些物种无性繁殖中的作用。我们的研究结果表明，在无性繁殖的网站预先存在的干细胞样细胞是负责形成的小植株。这些细胞被阻滞在细胞周期的不同阶段，并显示出不同的细胞形态特征和细胞计数。生长素和细胞分裂素在无性植株形成部位的积累表明了它们的重要功能，特别是对细胞周期的重新激活。这些差异可能会影响这些景天科物种的无性繁殖的模式和复杂性。此外，CUC 1和WUS的动态表达水平可能表明CUC 1在愈伤组织和芽分生组织的形成中起作用，而WUS仅与芽的诱导有关。
During asexual plant reproduction, cells from different organs can be reprogrammed to produce new individuals, a process that requires the coordination of cell cycle reactivation with the acquisition of other cellular morphological characteristics. However, the factors that influence the variety of asexual reproduction have not yet been determined. Here, we report on plantlet formation inKalanchoe daigremontiana,Graptopetalum paraguayense, andCrassula portulacea(Crassulaceae) and analyse the effect of initiating cells on asexual reproduction in these three species. Additionally, the roles ofWUSCHEL(WUS) andCUP-SHAPED COTYLEDON 1(CUC1) in the asexual reproduction of these species were analysed through qRT-PCR. Our results indicated that pre-existing stem cell-like cells at the sites of asexual reproduction were responsible for the formation of plantlets. These cells were arrested in different phases of the cell cycle and showed different cell morphological characteristics and cell counts. The accumulation of auxin and cytokinin at the sites of asexual plantlet formation indicated their important functions, particularly for cell cycle reactivation. These differences may influence the pattern and complexity of asexual reproduction in these Crassulaceae species. Additionally, the dynamic expression levels ofCUC1andWUSmay indicate thatCUC1functions in the formation of callus and shoot meristems; whereas,WUSwas only associated with shoot induction.