In vitro differentiation of dental epithelial, progenitor cells through epithelial-mesenchymal interactions
In vitro differentiation of dental epithelial, progenitor cells through epithelial-mesenchymal interactions
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DOI：
10.1016/j.archoralbio.2004.12.006
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发表时间：
2005-08-01
影响因子：
3
通讯作者：
Harada, H
中科院分区：
文献类型：
--
作者：
Morotomi, T;Kawano, S;Harada, H
在牙齿发育过程中，牙上皮祖细胞通过与神经嵴来源的间充质的顺序和相互作用而分化。然而，参与细胞分化的分子机制还不清楚。连续生长的牙齿可用于牙祖细胞分化的研究。在大鼠下切牙中，成釉细胞来源于牙上皮[成体干细胞隔室，称为“顶芽”。为了阐明成釉细胞的分化机制，我们设计了一个原代培养体系，并证实了牙上皮细胞通过与间充质细胞的相互作用而分化。细胞角蛋白被用作上皮细胞的标记物。细胞，内釉上皮神经生长因子受体p75。(IEE)细胞和用于成釉细胞的成釉蛋白。根尖芽细胞只能分化为IEE细胞，在牙乳头细胞存在的情况下，在10天内，分化为表达成釉蛋白的成釉细胞。有趣的是，在牙乳头细胞存在或不存在的情况下，IEE细胞可以瞬时增殖并分化为成釉细胞。这些结果表明，顶芽细胞可以通过与间充质细胞的相互作用进入成釉细胞谱系。另一方面，IEE细胞已经致力于分化成釉细胞。该培养体系可用于成釉细胞分化的研究。(C)2005 Elsevier Ltd.保留所有权利。
In developing teeth, dental epithelial progenitor cells differentiate through sequential and reciprocal interactions with neural-crest-derived mesenchyme. However, the molecular mechanisms involved in cell differentiation are not well understood. Continuously growing teeth are useful in the study of differentiation of dental progenitor cells. In rat lower incisors, amelobtasts originate from the dental epithelia[ adult stem cell compartment referred to as the 'apical bud'. To elucidate the mechanism of ameloblast differentiation, we designed a primary culture system and confirmed the differentiation of dental epithelial cells through interaction with mesenchymal cells. Cytokeratin was used as a marker for epithelial. cells, nerve growth factor receptor p75 for inner enamel epithelial. (IEE) cells, and ameloblastin for ameloblasts. The apical bud cells could only differentiate into IEE cells and, within 10 days, into ameloblasts expressing ameloblastin in the presence of dental papilla cells. Interestingly, the IEE cells could proliferate transiently and differentiate into ameloblasts in the presence or absence of dental papilla cells. These results suggest that apical bud cells can enter the ameloblast cell lineage through interaction with mesenchymal cells. IEE cells, on the other hand, are already committed to differentiate into amelobtasts. This culture system is useful in future studies of ameloblast differentiation. (C) 2005 Elsevier Ltd. All rights reserved.