Development and characterization of a binge drinking model in mice for evaluation of the immunological effects of ethanol
Development and characterization of a binge drinking model in mice for evaluation of the immunological effects of ethanol
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DOI：
10.1111/j.1530-0277.1996.tb01055.x
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发表时间：
1996-02-01
影响因子：
3.2
通讯作者：
Pruett, SB
中科院分区：
文献类型：
--
作者：
Carson, EJ;Pruett, SB
本研究描述了一种酗酒模型的开发和表征，其中通过管饲法向B6 C3 F1小鼠给予单剂量乙醇（EtOH），以3.0-7.0 g/kg的剂量给予EtOH后，随时间推移监测血液EtOH水平。峰值水平在0.2- 0.5%的范围内，在2-12小时内完全清除，观察到血液皮质酮水平显著增加，EtOH处理的8周龄小鼠的行为变化范围从无作用（3-4 g/kg）至重度共济失调（6-7 g/kg）。在16周龄的小鼠中，7 g/kg的剂量导致一些动物的翻正反射丧失和大多数其他动物的严重共济失调，临床化学结果未表明EtOH处理小鼠的一般生理/稳态系统发生生物学重要变化，但在7 g/kg剂量下有轻微肝损伤的迹象，因此，通过管饲法向B6 C3 F1小鼠施用EtOH产生行为变化、血液EtOH水平变化以及可能代表至少一些人类狂饮者的糖皮质激素水平变化。该模型被用来评估酗酒对抗体反应的影响，结果表明，该模型将是有用的，这样的研究。
This study describes the development and characterization of a binge drinking model in which a single dose of ethanol (EtOH) is administered by gavage to B6C3F1 mice, Blood EtOH levels were monitored over time after administration of EtOH at doses of 3.0-7.0 g/kg. Peak levels were in the range of 0.2-0.5%, and clearance was complete within 2-12 hr, Substantial increases in blood corticosterone levels were noted, Behavioral changes in EtOH-treated mice aged 8 weeks ranged from no effect (3-4 g/kg) to severe ataxia (6-7 g/kg). In mice aged 16 weeks, a dosage of 7 g/kg caused loss of the righting reflex in some animals and severe ataxia in most of the others, Clinical chemistry results did not indicate biologically important changes in general physiological/homeostatic systems in EtOH-treated mice, but there were indications of minor liver damage at the 7 g/kg dosage, Thus, administration of EtOH to B6C3F1 mice by gavage produces behavioral changes, changes in blood EtOH levels, and probably glucocorticoid levels representative of at least some human binge drinkers. The model was used to evaluate the effects of binge drinking on antibody responses, and the results indicate the model will be useful for such studies.