Systematic application of two-dimensional 1H nuclear-magnetic-resonance techniques for studies of proteins. 1. Combined use of spin-echo-correlated spectroscopy and J-resolved spectroscopy for the identification of complete spin systems of non-labile protons in amino-acid residues.
Systematic application of two-dimensional 1H nuclear-magnetic-resonance techniques for studies of proteins. 1. Combined use of spin-echo-correlated spectroscopy and J-resolved spectroscopy for the identification of complete spin systems of non-labile protons in amino-acid residues.
复制标题
二维 1H 核磁共振技术在蛋白质研究中的系统应用。
DOI：
10.1111/j.1432-1033.1981.tb05156.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
K. Wüthrich
中科院分区：
文献类型：
--
作者：
K. Nagayama;K. Wüthrich
这篇论文和下面的论文描述了最近开发的二维核磁共振技术在蛋白质研究中的实际应用。在本报告中，自旋回波相关光谱和二维J-分辨光谱用于确定完整的自旋系统的非不稳定，脂肪族质子的基本胰蛋白酶抑制剂。总的来说，41的58个脂肪族自旋系统在这种蛋白质中被确定;第一次在蛋白质中的所有甘氨酰残基的自旋系统已被确定在1H NMR光谱。结合下面的文章，目前的数据产生新的个人分配的许多氨基酸残基，并提供了一个新的途径，基于精确测量的自旋-自旋耦合常数在二维J-分辨光谱，研究静态和动态方面的变化，蛋白质构象之间的单晶和溶液，或不同的溶剂和温度的条件。
This and the following paper describe the practical application of recently developed, two-dimensional nuclear magnetic resonance techniques for studies of proteins. In the present report spin-echo-correlated spectroscopy and two-dimensional J-resolved spectroscopy are used to identify complete spin systems of non-labile, aliphatic protons in the basic pancreatic trypsin inhibitor. Overall, 41 out of the 58 aliphatic spin systems in this protein were identified; for the first time the spin systems of all the glycyl residues in a protein have been identified in the 1H NMR spectrum. Combined with the following paper, the present data yield new individual assignments for numerous amino acid residues and provide a new avenue, based on accurate measurements of spin-spin coupling constants in the two-dimensional J-resolved spectra, for studying changes of static and dynamic aspects of protein conformation between single crystals and solution, or between different conditions of solvent and temperature.