Ligand-Induced Receptor-like Kinase Complex Regulates Floral Organ Abscission in Arabidopsis.
Ligand-Induced Receptor-like Kinase Complex Regulates Floral Organ Abscission in Arabidopsis.
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DOI：
10.1016/j.celrep.2016.01.023
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发表时间：
2016-02-16
期刊：
影响因子：
8.8
通讯作者：
Shan L
中科院分区：
文献类型：
--
作者：
Meng X;Zhou J;Tang J;Li B;de Oliveira MVV;Chai J;He P;Shan L
脱落是一个发育过程，使植物摆脱不需要的器官。在拟南芥中，花器官的发育受肽配体IDA、受体样激酶（RLK）HAE和HSL 2以及下游MAP激酶（MAPK）级联组成的信号通路调节。然而，人们对配体-受体对与细胞内信号传导之间的分子联系知之甚少。在这里，我们报告说，SERK家族RLKs功能冗余调节花器官脱落下游的IDA和上游的MAPK级联。IDA诱导HAE/HSL 2和SERKs的异源二聚化，其彼此转磷酸化。介导其与免疫受体FLS 2和油菜素类固醇受体BRI 1相互作用的SERK 3残基也是IDA诱导的HAE/HSL 2-SERK 3相互作用所需的，表明SERKs在感知IDA中充当HAE/HSL 2的共受体。因此，我们的研究揭示了花器官脱落的信号激活机制，通过IDA诱导的HAE/HSL 2-SERK复合物的形成伴随转磷酸化。
Abscission is a developmental process that enables plants to shed unwanted organs. In Arabidopsis, the floral organ abscission is regulated by a signaling pathway consisting of the peptide ligand IDA, the receptor-like kinases (RLKs) HAE and HSL2, and a downstream MAP kinase (MAPK) cascade. However, little is known about the molecular link between ligand-receptor pairs and intracellular signaling. Here, we report that the SERK family RLKs function redundantly in regulating floral organ abscission downstream of IDA and upstream of the MAPK cascade. IDA induces heterodimerization of HAE/HSL2 and SERKs, which transphosphorylate each other. The SERK3 residues mediating its interaction with the immune receptor FLS2 and the brassinosteroid receptor BRI1 are also required for IDA-induced HAE/HSL2-SERK3 interaction, suggesting SERKs serve as co-receptors of HAE/HSL2 in perceiving IDA. Thus, our study reveals the signaling activation mechanism in floral organ abscission by IDA-induced HAE/HSL2-SERK complex formation accompanied by transphosphorylation.