Relaxin immunoactivity in plasma during the reproductive cycle of the female guinea pig.
Relaxin immunoactivity in plasma during the reproductive cycle of the female guinea pig.
复制标题
雌性豚鼠生殖周期期间血浆中松弛素的免疫活性。
DOI：
10.1095/biolreprod24.2.405
复制
发表时间：
1981
影响因子：
3.6
通讯作者：
G. Bryant
中科院分区：
文献类型：
--
作者：
S. Boyd;J. Kendall;N. Mento;G. Bryant
使用同源猪松弛素放射免疫测定法在从怀孕中期到分娩的动情周期以及急性哺乳期间测量豚鼠的血浆松弛素免疫活性。每隔一段时间从留置的颈静脉导管中采集样本。怀孕期间发现的峰值水平为 12 ng/ml，高于动情周期和哺乳期间的峰值水平 (4-5 ng/ml)。在发情周期期间，松弛素水平在发情间和发情间早期较低，而在发情间后期和阴道膜打开之前的发情前期较高。在妊娠后期，免疫反应性恢复素的间歇性分泌很常见，并且这种分泌的时间各不相同。在一些豚鼠中，松弛素水平在怀孕中后期增加；另一些则仅在分娩前或妊娠中晚期出现上升。数据的光谱分析揭示了几种动物的结果存在明显的周期性，但其他动物则没有显示出周期性的证据。最常见的时间是 18 小时。在大多数（但不是全部）动物中，通过触诊确定的耻骨韧带延长开始之前是松弛素产生的时期。没有证据表明哺乳对增加该物种的松弛素分泌有急性作用；事实上，哺乳降低了松弛素的免疫活性，对照动物的平均水平显着高于哺乳幼仔的动物。
Plasma relaxin immunoactivity was measured in guinea pigs during the estrous cycle from midpregnancy to parturition as well as during acute suckling using a homologous porcine relaxin radioimmunoassay. Samples were collected at frequent intervals from an indwelling jugular vein catheter. The peak levels found during pregnancy, \.‘12 ng/ml, were higher than the peak levels during the estrous cycle and suckling (4-5 ng/ml). During the estrous cycle, relaxin levels were low at estrus and early diestrus and were high at late diestrus and proestrus preceeding the opening of the vaginal membrane. During late pregnancy, episodic secretion of immunoreactive retaxin was common and the timing of this secretion varied. In some guinea pigs, relaxin levels increased in mid to late pregnancy; others showed only a rise before parturition or only the mid to late pregnancy rise. Spectral analysis of the data revealed clear periodicities in the results from several animals but others showed no evidence of periodicity. The most common period was 18 h. In most animals, but not all, the onset of lengthening of the pubic ligament as determined by palpation was preceded by a period of relaxin production. There was no evidence for an acute effect of suckling to elevate relaxin secretion in this species; indeed suckling decreased relaxin immunoactivity with mean levels of control animals significantly higher than those of animals suckling their young.