The involvements of calcium-dependent protein kinases and catechins in tea plant [Camellia sinensis (L.) O. Kuntze] cold responses
The involvements of calcium-dependent protein kinases and catechins in tea plant [Camellia sinensis (L.) O. Kuntze] cold responses
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茶树 [Camellia sinensis (L.) O. Kuntze] 冷反应中钙依赖性蛋白激酶和儿茶素的参与
DOI：
10.1016/j.plaphy.2019.09.005
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发表时间：
2019-10-01
影响因子：
6.5
通讯作者：
Wang, Xinchao
中科院分区：
文献类型：
--
作者：
Ding, Changqing;Lei, Lei;Wang, Xinchao
温度是限制茶树生长和茶叶生产的重要环境因子之一。以往的研究表明，Ca ~（2+）和ROS信号在茶树冷驯化过程中起着重要作用。本研究鉴定了26个CsCPK转录本，分析了它们的系统发育和序列特征，并检测了它们的转录本，以监测Ca 2+信号转导状态。组织特异性表达谱表明，大多数CsCPK基因在测试组织中组成型表达，这表明它们可能在发育中的作用。冷沿着钙抑制剂的测定提示CsCPKs是重要的冷调节因子，CsCPK 30/5/4/9可能是其中的关键成员。此外，LaCl 3或EGTA预处理可导致Ca 2+信号转导受损，冷反应网络受损，但较高的儿茶素积累揭示了它们在冷反应中的潜在积极作用。这些结果表明，茶树中的儿茶素等次生代谢产物可能形成了一个与Ca 2+信号状态密切相关的替代冷响应网络。
Temperature is one of the most important environmental factors limiting tea plant growth and tea prodUction. Previously we reported that both Ca2+ and ROS signals play important roles in tea plant cold acclimation. Here, we identified 26 CsCPK transcripts, analyzed their phylogenetic and sequence characters, and detected their transcriptions to monitor Ca2+ signaling status. Tissue-specific expression profiles indicated that most CsCPK genes were constitutively expressed in tested tissues, suggesting their possible roles in development. Cold along with calcium inhibitor assays suggested that CsCPKs are important cold regulators and CsCPK30/5/4/9 maybe the key members. Moreover, LaCl3 or EGTA pre-treatment could result in impaired Ca2+ signaling and compromised cold-responding network, but higher catechins accumulation revealed their potential positive roles in cold responses. Those findings indicated that catechins and other secondary metabolites in tea plant may form an alternative cold-responding network that closely correlated with Ca2+ signaling status.