Localization and osteoblastic differentiation potential of neural crest-derived cells in oral tissues of adult mice.
Localization and osteoblastic differentiation potential of neural crest-derived cells in oral tissues of adult mice.
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DOI：
10.1016/j.bbrc.2015.07.106
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发表时间：
2015-09
影响因子：
3.1
通讯作者：
Miki Ono;T. Suzawa;M. Takami;G. Yamamoto;Tomohiko Hosono;A. Yamada;D. Suzuki;K. Yoshimura;J. Watahiki;R. Hayashi;S. Arata;K. Mishima;K. Nishida;N. Osumi;K. Maki;R. Kamijo
中科院分区：
文献类型：
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作者：
Miki Ono;T. Suzawa;M. Takami;G. Yamamoto;Tomohiko Hosono;A. Yamada;D. Suzuki;K. Yoshimura;J. Watahiki;R. Hayashi;S. Arata;K. Mishima;K. Nishida;N. Osumi;K. Maki;R. Kamijo
在胚胎中，神经嵴细胞从融合神经管的背侧区域出现，并在组织中迁移，分化成各种类型的细胞，包括成骨细胞。在成人中，神经冠源性细胞（ncdc）的亚群以干细胞的形式存在，被认为是再生医学策略的有用细胞来源。大量研究表明，具有神经嵴起源的干细胞持续存在于成年期，特别是那些在哺乳动物颅面腔内的干细胞。然而，它们在成人中的分布以及向成骨细胞分化的能力尚不完全清楚。为了分析ncdc在成人口腔组织中的精确分布和特征，我们利用已建立的双转基因（P0-Cre/CAG-CAT-EGFP）小鼠系，其中ncdc在其一生中表达绿色荧光蛋白（GFP）。成年小鼠的上颚、牙龈、舌头和颊粘膜组织中，gfp阳性细胞像岛一样分散，从后者分离出来的细胞在低贴壁条件下形成球体，这是由干细胞组成的典型细胞团。此外，茜素红染色显示，在骨形态发生蛋白(BMP)-2存在的情况下，gfp阳性细胞的碱性磷酸酶（成骨细胞分化的标记酶）活性和矿化明显增加。这些结果表明ncdc存在于各种成人口腔组织中，并具有向成骨细胞分化的潜力。成人非传染性疾病可能是骨再生策略的有用细胞来源。
In embryos, neural crest cells emerge from the dorsal region of the fusing neural tube and migrate throughout tissues to differentiate into various types of cells including osteoblasts. In adults, subsets of neural crest-derived cells (NCDCs) reside as stem cells and are considered to be useful cell sources for regenerative medicine strategies. Numerous studies have suggested that stem cells with a neural crest origin persist into adulthood, especially those within the mammalian craniofacial compartment. However, their distribution as well as capacity to differentiate into osteoblasts in adults is not fully understood. To analyze the precise distribution and characteristics of NCDCs in adult oral tissues, we utilized an established line of double transgenic (P0-Cre/CAG-CAT-EGFP) mice in which NCDCs express green fluorescent protein (GFP) throughout their life. GFP-positive cells were scattered like islands throughout tissues of the palate, gingiva, tongue, and buccal mucosa in adult mice, with those isolated from the latter shown to form spheres, typical cell clusters composed of stem cells, under low-adherent conditions. Furthermore, GFP-positive cells had markedly increased alkaline phosphatase (a marker enzyme of osteoblast differentiation) activity and mineralization as shown by alizarin red staining, in the presence of bone morphogenetic protein (BMP)-2. These results suggest that NCDCs reside in various adult oral tissues and possess potential to differentiate into osteoblastic cells. NCDCs in adults may be a useful cell source for bone regeneration strategies.