Fine genetic mapping of a locus controlling short internode length in melon (Cucumis melo L.)
Fine genetic mapping of a locus controlling short internode length in melon (Cucumis melo L.)
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DOI：
10.1007/s11032-014-0088-1
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发表时间：
2014-10-01
影响因子：
3.1
通讯作者：
Park, Younghoon
中科院分区：
文献类型：
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作者：
Hwang, Jihyun;Oh, Juyoul;Park, Younghoon
甜瓜（Cucumis梅洛L.; 2n = 2x = 24）可能具有商业重要性，因为它们可以有助于促进集中坐果，并且可以以比标准藤蔓类型更高的植物密度种植。本研究旨在确定甜瓜矮化突变系PNU-D1（C.梅洛cantalupensis）。PNU-D1与自交系野生型甜瓜PNU-WT1（C.梅洛agrestis），然后将所得的F-1后代自花授粉以产生分离为矮化和蔓生植物类型的F-2群体。在温室条件下测定的F-2后代的主茎长度表明，该群体的矮秆性受一对隐性基因mdw1控制。为了确定与mdw1相关的染色体位置，利用94个F-2后代构建了基于简单重复序列（SSR）的遗传连锁图谱。利用分布在15个连锁群上的76个SSR标记，将mdw1定位于第7染色体上，分子量为462.84 cM。利用这些矮秆相关基因，对1，194个F-2后代进行了mdw1基因组区域的精细遗传作图，确定了mdw1与黄瓜紧凑生长习性（cp）候选基因细胞分裂素氧化酶基因之间的遗传距离为1.7 cM。候选基因ERECTA（丝氨酸/苏氨酸激酶）和UBI（泛素）也被映射到基因组区域侧翼mdw1在0.6和1.2 cM的距离，分别。
Compact and dwarfing vining habits in melon (Cucumis melo L.; 2n = 2x = 24) may have commercial importance since they can contribute to the promotion of concentrated fruit set and can be planted in higher plant densities than standard vining types. A study was designed to determine the genetics of dwarfism associated with a diminutive (short internodes) melon mutant line PNU-D1 (C. melo ssp. cantalupensis). PNU-D1 was crossed with inbred wild-type melon line PNU-WT1 (C. melo ssp. agrestis), and resultant F-1 progeny were then self-pollinated to produce an F-2 population that segregated as dwarf and vining plant types. Primary stem length of F-2 progeny assessed under greenhouse conditions indicated that a single recessive gene, designated mdw1, controlled dwarfism in this population. To identify the chromosomal location associated with mdw1, an simple sequence repeat (SSR)-based genetic linkage map was constructed using 94 F-2 progeny. Using 76 SSR markers positioned on 15 linkage groups spanning 462.84 cM, the location of mdw1 was localized to Chromosome 7. Using the putative dwarfing-associated genes, fine genetic mapping of the mdw1 genomic region was facilitated with 1,194 F-2 progeny that defined the genetic distance between mdw1 and cytokinin oxidase gene, a candidate gene for compact growth habit (cp) in cucumber, to be 1.7 cM. The candidate gene ERECTA (serin/threonine kinase) and UBI (ubiquitin) were also mapped to genomic regions flanking mdw1 at distances of 0.6 and 1.2 cM, respectively.