Developmental changes of thyroid hormones in the fathead minnow, Pimephales promelas
Developmental changes of thyroid hormones in the fathead minnow, Pimephales promelas
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DOI：
10.1016/j.ygcen.2004.07.004
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发表时间：
2004-10-01
影响因子：
2.7
通讯作者：
Brown, JA
中科院分区：
文献类型：
--
作者：
Crane, HM;Pickford, DB;Brown, JA
黑头呆鱼被广泛用于生态毒理学研究，这种研究已经开始关注甲状腺轴的潜在破坏。然而，甲状腺素（T-4）和3，5，3 '-三碘甲状腺原氨酸（T-3）的正常水平及其发育模式尚不清楚。为了提供这些基线数据，开发并验证了用于从血浆或整条鱼中提取后分析T-4和T-3的放射免疫测定法。雌鱼的血浆T-4水平始终高于雄鱼。对发育过程中甲状腺激素的分析表明，在孵化前阶段，T-4和T-3都显着上升，表明胚胎产生两种甲状腺激素。孵化后，T-4和T-3的整体含量在早期发育中显著增加，分别在孵化后16天和9天达到峰值，此时T-4达到32.88 +/- 3.30 ng g（-1）体重，T-3达到24.17 +/- 3.15 ng g（-1）体重。甲状腺激素随后在发育后期下降至低平台，T-4和T-3的体重分别约为5 ng g（-1）和2 ng g（-1）。这些数据表明，甲状腺激素在早期发育过程中的突出作用时，我们预测，甲状腺干扰物的生态毒理学效应将是最显着的。(C)2004爱思唯尔公司All rights reserved.
The fathead minnow is widely used in ecotoxicological studies and such investigations have begun to focus on potential disruption of the thyroid axis. However, normal levels of thyroxine (T-4) and 3,5,3'-triiodothyronine (T-3) and their developmental patterns are unknown. To provide these baseline data, radioimmunoassays were developed and validated for analysis of T-4 and T-3 after extraction from plasma or whole fish. Female fish showed consistently higher plasma levels of T-4 than male fish. Analysis of thyroid hormones during development showed a significant rise in both T-4 and T-3 during the pre-hatch period, indicating embryonic production of both thyroid hormones. After hatching, whole-body content of both T-4 and T-3 significantly increased in early development, peaking at 16 days post-hatch (dph) and 9 dph, respectively, when T-4 reached 32.88 +/- 3.30 ng g(-1) body weight and T-3 reached 24.17 +/- 3.15 ng g(-1) body weight. Thyroid hormones subsequently declined to a low plateau in later development with approximately 5 ng g(-1) body weight T-4 and 2 ng g(-1) body weight T-3. These data suggest a prominent role for thyroid hormones in early developmental processes when we predict that the ecotoxicological effects of thyroid disrupters will be most significant. (C) 2004 Elsevier Inc. All rights reserved.