Growth interaction in vivo between tumor subpopulations derived from a single mouse mammary tumor.
Growth interaction in vivo between tumor subpopulations derived from a single mouse mammary tumor.
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DOI：
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发表时间：
1980-11
期刊：
影响因子：
11.2
通讯作者：
B. Miller;F. Miller;J. Leith;G. Heppner
中科院分区：
文献类型：
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作者：
B. Miller;F. Miller;J. Leith;G. Heppner
我们的实验室此前已从 BALB/cfC3H 小鼠的单个自发乳腺肿瘤中分离出多个肿瘤细胞群，并将它们作为独立的亚系在组织培养中建立。这些亚群根据许多标准而有所不同，包括生长参数和肿瘤相关抗原的表达。我们通过将相同或不同亚系的细胞悬液注射到 BALB/cfC3H 或 BALB/c 小鼠的相对侧腹，测试了其中几个亚群的体内相互作用。某些亚群的生长特征因另一侧不同亚群的存在而改变。为了了解相互作用的机制，我们选择了两个亚群（410和168）进行进一步研究。在 BALB/cfC3H 小鼠中，一侧胁腹上 410 系肿瘤的存在抑制了另一侧侧的 410 和 168 肿瘤。 168系肿瘤不抑制410或168系肿瘤。细胞系410的抑制作用似乎是免疫学的，因为(a)通过在细胞系168之前几周注射细胞系410来增加抑制作用，(b)在肿瘤细胞注射前2天接受400拉德X射线照射的小鼠中，抑制作用被消除，(c)通过植入随后手术切除细胞系410但不是细胞系168，可以使小鼠对细胞系410和细胞系168肿瘤产生抗性，并且(d) 在 Winn 测定中，耐药性可以通过来自 410 系致敏小鼠的淋巴结细胞适应性转移。因此，对肿瘤相关抗原的免疫可能是异质肿瘤细胞相互作用的一种方式。
Our laboratory has previously isolated several tumor cell populations from a single, spontaneously arising mammary tumor of a BALB/cfC3H mouse and established them in tissue culture as independent sublines. These subpopulations differ according to many criteria including growth parameters and expression of tumor-associated antigens. We have tested the interaction in vivo of several of these subpopulations by injecting cell suspensions of the same or different sublines into opposite flanks of BALB/cfC3H or BALB/c mice. The growth characteristics of certain subpopulations were altered by the presence of a different subpopulation on the opposite side. In order to understand the mechanism of interaction, we chose two subpopulations (410 and 168) for further study. In BALB/cfC3H mice, the presence of line 410 tumors on one flank inhibited both 410 and 168 tumors on the other flank. Line 168 tumors did not inhibit either 410 or 168 tumors. The inhibitory effect of line 410 appeared to be immunological, since (a) it was increased by injecting line 410 several weeks before line 168, (b) it was abrogated in mice subjected to 400-rad X-irradiation 2 days prior to tumor cell injection, (c) mice could be made resistant to both line 410 and line 168 tumors by implantation followed by surgical removal of line 410 but not of line 168, and (d) resistance could be adaptively transferred with lymph node cells from line 410-sensitized mice in Winn assays. Thus, immunity to tumor-associated antigens may be one way by which cells of a heterogeneous tumor can interact.