Tim-3-Galectin-9 pathway involves the suppression induced by CD4+CD25+ regulatory T cells
Tim-3-Galectin-9 pathway involves the suppression induced by CD4+CD25+ regulatory T cells
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DOI：
10.1016/j.imbio.2008.10.007
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发表时间：
2009-05-01
期刊：
影响因子：
2.8
通讯作者：
Chen, Zhonghua Klaus
中科院分区：
文献类型：
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作者：
Wang, Feng;Wan, Lagen;Chen, Zhonghua Klaus
CD4(+)CD25(+)调节性T细胞(Treg)被认为作为免疫介导反应的抑制因子发挥着关键作用。已经在各种条件下研究了动物和患有自身免疫性疾病、过敏性疾病或致癌性疾病的患者的这种抑制机制。然而，CD4(+)CD25(+) Tregs 的精确抑制模式仍不清楚。在本报告中，Tim-3-Galectin-9 通路被探索为 Tregs 抑制和细胞毒性的机制之一。在这里，我们通过逆转录聚合酶链反应（RT-PCR）和Western blot证明Galectin-9在CD4（+）CD25（+）Tregs上表达。有趣的是，体外阻断Tim-3-Galectin-9通路会导致Tregs的抑制活性明显降低，Th1细胞因子水平升高。此外，阻断Tim-3-Galectin-9通路可消除体内CD4(+)CD25(+) Tregs诱导的同种异体皮肤移植物的延长存活期。我们的结果表明 Tim-3-Galectin-9 通路涉及 CD4(+)CD25(+) Tregs 的功能。 (C) 2008 Elsevier GrnbH。版权所有。
CD4(+)CD25(+) regulatory T cells (Tregs) are considered to play a key role as suppressors of immune-mediated reactions. The mechanisms of this suppression in animals and patients with autoimmune, allergic or oncogenic diseases have been investigated under various conditions. However, the precise mode of suppression by CD4(+)CD25(+) Tregs is still not clear. In this report, Tim-3-Galectin-9 pathway was explored as one of the mechanisms for the suppression and cytotoxicity induced by Tregs. Here, we demonstrated that Galectin-9 was expressed on CD4(+)CD25(+) Tregs by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Interestingly, blocking Tim-3-Galectin-9 pathway resulted in an obvious decreased suppression activity of Tregs and enhanced Th1 cytokine level in vitro. Furthermore, blocking Tim-3-Galectin-9 pathway negated prolonged survival of allogeneic skin grafts induced by CD4(+)CD25(+) Tregs in vivo. Our results suggest that Tim-3-Galectin-9 pathway involves the function of CD4(+)CD25(+) Tregs. (C) 2008 Elsevier GrnbH. All rights reserved.