The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.
The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.
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DOI：
10.1083/jcb.200609153
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发表时间：
2007-03-26
影响因子：
7.8
通讯作者：
Koshland, Douglas
中科院分区：
文献类型：
--
作者：
Yu, Hong-Guo;Koshland, Douglas
同源分离在第一次减数分裂需要适当的空间调节姐妹染色单体的凝聚力和溶解沿着染色体臂，但在着丝粒区域的保护。这种保护需要保守的MEI-S332/Sgo 1蛋白定位于着丝粒区域，并通过结合其调节亚基Rts 1来募集PP 2A磷酸酶。然后，中心Rts 1/PP 2A可能通过使蛋白质复合物粘附素去磷酸化来局部防止粘附溶解。我们表明，极光B激酶在酿酒酵母（Ipl 1）也是必不可少的保护减数分裂着丝粒凝聚力。结合先前在果蝇中的研究，极光B激酶的减数分裂功能似乎在真核生物中是保守的。此外，我们发现，Sgo 1招聘Ipl 1的着丝粒区域。在缺乏Ipl 1的情况下，Rts 1最初可以结合到着丝粒区域，但在后期I发作后从这些区域消失。我们认为，着丝粒Ipl 1确保继续着丝粒存在的活性Rts 1/PP 2A，这反过来又局部保护cohesin和凝聚力。
Homologue segregation during the first meiotic division requires the proper spatial regulation of sister chromatid cohesion and its dissolution along chromosome arms, but its protection at centromeric regions. This protection requires the conserved MEI-S332/Sgo1 proteins that localize to centromeric regions and also recruit the PP2A phosphatase by binding its regulatory subunit, Rts1. Centromeric Rts1/PP2A then locally prevents cohesion dissolution possibly by dephosphorylating the protein complex cohesin. We show that Aurora B kinase in Saccharomyces cerevisiae (Ipl1) is also essential for the protection of meiotic centromeric cohesion. Coupled with a previous study in Drosophila melanogaster, this meiotic function of Aurora B kinase appears to be conserved among eukaryotes. Furthermore, we show that Sgo1 recruits Ipl1 to centromeric regions. In the absence of Ipl1, Rts1 can initially bind to centromeric regions but disappears from these regions after anaphase I onset. We suggest that centromeric Ipl1 ensures the continued centromeric presence of active Rts1/PP2A, which in turn locally protects cohesin and cohesion.