A protein linkage map of Escherichia coli bacteriophage T7
A protein linkage map of Escherichia coli bacteriophage T7
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DOI：
10.1038/ng0196-72
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发表时间：
1996-01-01
期刊：
影响因子：
30.8
通讯作者：
Fields, S
中科院分区：
文献类型：
--
作者：
Bartel, PL;Roecklein, JA;Fields, S
基因组测序项目预测了大量的新蛋白质，它们与其他蛋白质的相互作用必须介导细胞过程的功能。为了分析这些网络，我们在全基因组范围内使用酵母双杂交系统来识别大肠杆菌噬菌体T7蛋白质之间的25种相互作用。其中有一组六种相互作用，连接在DNA复制和DNA包装中起作用的蛋白质。值得注意的是，两个基因，排列成一个完全重叠另一个，并使用不同的阅读框架，编码相互作用的蛋白质。几个相互作用反映了同一多肽的不同结构域的分子内协会，这表明双杂交测定可能是有用的蛋白质折叠的分析。这种蛋白质-蛋白质相互作用的全局方法可能适用于分析更复杂的基因组，其序列正在变得可用。
Genome sequencing projects are predicting large numbers of novel proteins, whose interactions with other proteins must mediate the function of cellular processes. To analyse these networks, we used the yeast two-hybrid system on a genome-wide scale to identify 25 interactions among the proteins of Escherichia coli bacteriophage T7. Among these is a set of six interactions connecting proteins that function in DNA replication and DNA packaging. Remarkably, two genes, arranged such that one entirely overlaps the other and uses a different reading frame, encode interacting proteins. Several of the interactions reflect intramolecular associations of different domains of the same polypeptide, suggesting that the two-hybrid assay may be useful in the analysis of protein folding. This global approach to protein-protein interactions may be applicable to the analysis of more complex genomes whose sequences are becoming available.