A novel FT-IR spectroscopic method based on lipid characteristics for qualitative and quantitative analysis of animal-derived feedstuff adulterated with ruminant ingredients
A novel FT-IR spectroscopic method based on lipid characteristics for qualitative and quantitative analysis of animal-derived feedstuff adulterated with ruminant ingredients
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DOI：
10.1016/j.foodchem.2017.05.011
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发表时间：
2017-12-15
期刊：
影响因子：
8.8
通讯作者：
Liu, Xian
中科院分区：
文献类型：
--
作者：
Gao, Fei;Zhou, Simiao;Liu, Xian
本研究的目的是探讨傅立叶变换红外光谱（FT-IR）鉴别掺假动物源性饲料的能力。共18种原料肉骨粉（包括9种非反刍动物肉骨粉和9种反刍动物肉骨粉）被混合，以获得81种特定比例（1-35%）的二元混合物。采用傅立叶变换红外光谱法结合化学计量学分析了脂肪的光谱特征。FT-IR谱图随掺杂浓度的变化而变化。结果表明，根据脂肪特性可以成功地鉴别反刍动物掺假。建立了反刍动物掺假的偏最小二乘回归模型，模型的有效性（R-P（2）> 0.90）。此外，通过计算脂肪酸中C=C/C=O和C=C/C-H（CH 2）的比值以及脂肪酸中C-H（-CH 2）的数量，发现反式脂肪酸含量和不饱和度的差异是影响FT-IR光谱法判断掺伪的主要因素。(C)2017由Elsevier Ltd.出版
The objective of this study was to explore the ability of Fourier transform infrared (FT-IR) spectroscopy to authenticate adulterated animal-derived feedstuff. A total of 18 raw meat and bone meals (MBMs), including 9 non-ruminant MBMs and 9 ruminant MBMs, were mixed to obtain 81 binary mixtures with specific proportions (1-35%). Lipid spectral characteristics were analyzed by FT-IR spectroscopy combined with chemometrics. Changes in FT-IR spectra were observed as adulterant concentration was varied. The results illustrate ruminant adulteration can be successfully distinguished based on lipid characteristics. PLS model was established to quantify ruminant adulteration, which was shown to be valid (R-P(2) > 0.90). Furthermore, the ratios of C=C/C=O and C=C/C-H(CH2), as well as the number of C-H (-CH2) in the fatty acids of adulterated lipids, were calculated, which showed that differences in the trans fatty acid content and the degree of unsaturation were the main contributors to determination of adulteration based on FT-IR spectroscopy. (C) 2017 Published by Elsevier Ltd.