Negative Impact of Proteinuria on Circulating Myeloid Dendritic Cells
Negative Impact of Proteinuria on Circulating Myeloid Dendritic Cells
复制标题
蛋白尿对循环骨髓树突状细胞的负面影响
DOI：
10.1007/s10157-019-01724-7
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发表时间：
2019
影响因子：
2.3
通讯作者：
Takashi Yokoo
中科院分区：
文献类型：
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作者：
Masato Ikeda 1 ;Risa Terashima 2 ;Taku Yamada 3 ;Masahiro Suyama 3 ;Shinya Yokote 3 ;Masatsugu Nakao 2 ;Izumi Yamamoto 2 ;Keita Hirano 2 ;Hideo Okonogi 2 ;Hiroyasu Yamamoto 2 ;Takashi Yokoo
背景：循环树突状细胞（DC）的绝对数量（abs）的减少和靶器官的募集已经有报道，但蛋白尿水平是否与循环树突状细胞（DC）的绝对数量（abs）减少有关尚未明确。方法：我们对2007-2010年在我院接受肾活检的210例21-96岁肾脏疾病患者进行横断面研究。为了准确性，蛋白尿水平是通过24小时收集入院患者的尿液来彻底测量的。采用三色荧光活化细胞分选法（FACS）测定总DCs （tDCs）、髓样DCs （mDCs）和浆细胞样DCs （pDCs）的抗体。根据各DC抗体的四分位数将患者分为四组，并进行单因素方差分析和多变量调整回归分析。结果分位数分析显示，随着血液中mDC抗体的增加，每日蛋白尿水平降低，mDC抗体患者的平均蛋白尿水平（g/d）分别为2.45、1.68、1.68、1.10，四分位数分别≤445、< 686、< 907、≥907个细胞/102µL （p= 0.0277）。多变量校正回归分析显示mDC抗体与蛋白尿呈负相关（95% CI为- 57.0 ~ - 8.5），与男性性别呈正相关（95% CI为66.2 ~ 250.5）。此外，研究还显示，原发性胆管癌与肾小球滤过率（eGFR） （95% CI 0.14-2.67）和c反应蛋白（95% CI - 49.4 - - 9.9）之间存在独立关联，tDCs与男性性别（95% CI 54.5-253.6）和c反应蛋白（95% CI - 80.5 - - 13.4）之间存在独立关联。结论我们首次报道了血液循环中的mDC抗体与蛋白尿水平呈负相关。
BackgroundA decrease in absolute numbers (abs.) of circulating dendritic cells (DCs) and recruitment into target organs has been reported, but whether the level of proteinuria associates with circulating DC abs. has not been clarified.MethodsWe conducted a cross-sectional study of 210 patients with kidney disease aged 21–96 years who were admitted to our hospital for kidney biopsy in 2007–2010. For accuracy, the level of proteinuria was thoroughly measured by 24-h urine collection from patients in their admitted condition. The abs. of total DCs (tDCs), myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) was measured by three-color fluorescence-activated cell sorting (FACS). Patients were divided into four groups based upon the quartile of each DC abs. and one-way ANOVA, and multivariable-adjusted regression analyses were performed.ResultsQuantile analysis showed that the level of daily proteinuria decreased with increasing blood mDC abs., with mean proteinuria levels (g/day) of 2.45, 1.68, 1.68, 1.10 for those in mDC abs. quartiles ≤ 445, < 686, < 907, ≥ 907 cells/102µL (p= 0.0277), respectively. Multivariate-adjusted regression analysis revealed that the mDC abs. was negatively associated with proteinuria (95% CI − 57.0 to − 8.5) and positively associated with male gender (95% CI 66.2–250.5). Independent associations were also shown between pDCs abs. and estimated glomerular filtration rate (eGFR) (95% CI 0.14–2.67) and C-reactive protein (95% CI − 49.4 to − 9.9) and between tDCs abs. and male gender (95% CI 54.5–253.6) and C-reactive protein (95% CI − 80.5 to − 13.4).ConclusionWe first reported that circulating mDC abs. has a negative association with the level of proteinuria.