mRNAStab-a web application for mRNA stability analysis
mRNAStab-a web application for mRNA stability analysis
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DOI：
10.1093/bioinformatics/btt040
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发表时间：
2013-03-15
期刊：
影响因子：
5.8
通讯作者：
Arnau, Vicente
中科院分区：
文献类型：
--
作者：
Alic, Andrei;Perez-Ortin, Jose E.;Arnau, Vicente
真核基因表达在转录和mRNA降解水平上都受到调节。功能基因组学方法的实现允许同时测量数千种mRNA的转录（TR）和降解（DR）速率，这是该领域的巨大进步。确定mRNA稳定性的最佳方法之一是基因组运行（GRO）。它允许在细胞动态响应期间测量DR、TR和mRNA水平。在这里，我们提供了一个软件包，它提供了改进的算法，用于在动态GRO实验中确定mRNA的稳定性。可用性和实施：程序mRNAStab可在http://mRNAStab.uv.es/上免费访问。mRNAStab是用C、PHP和R编写的。联系方式：vicente.arnau@uv. es补充信息：补充数据可在Bioinformatics online获得。
Eukaryotic gene expression is regulated both at the transcription and the mRNA degradation levels. The implementation of functional genomics methods that allow the simultaneous measurement of transcription (TR) and degradation (DR) rates for thousands of mRNAs is a huge improvement in this field. One of the best established methods for mRNA stability determination is genomic run-on (GRO). It allows the measurement of DR, TR and mRNA levels during cell dynamic responses. Here, we offer a software package that provides improved algorithms for determination of mRNA stability during dynamic GRO experiments.Availability and implementation: The program mRNAStab is freely accessible at http://mRNAStab.uv.es/. mRNAStab is written in C, PHP and R.Contact: vicente.arnau@uv.esSupplementary information: Supplementary data are available at Bioinformatics online.