Ultra-sensitive detection of IgE using biofunctionalized nanoparticle-enhanced SPR
Ultra-sensitive detection of IgE using biofunctionalized nanoparticle-enhanced SPR
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DOI：
10.1016/j.talanta.2010.03.036
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发表时间：
2010-06-15
期刊：
影响因子：
6.1
通讯作者：
Lee, Hye Jin
中科院分区：
文献类型：
--
作者：
Kim, Suhee;Lee, Jaeyoung;Lee, Hye Jin
本文介绍了一种基于纳米粒子增强表面等离子体共振(SPR)的超灵敏表面检测方法，用于检测免疫球蛋白E(IgE)蛋白，该方法可能用于变态反应性疾病的诊断。首次用SPR研究了两种不同的探针，即抗IgE和IgE特异性适配子，它们能与不同表位的IgE特异性相互作用。测得IgE与抗-IgE和IgE特异性适配子相互作用的朗缪尔吸附系数(K-ADS)分别为2.0(+/-0.22)×10(8)M-1和2.2(+/-0.20)×10(8)M-1。分别进行了分析。简单吸附在抗-IgE或IgE特异性适配子上的SPR检测下限约为1 nm。为了改善免疫球蛋白E的SPR检测信号，设计了两种不同的利用表面形成的生物功能化金纳米颗粒夹心络合物(Au-NPS)的检测方法，并比较了两种方法的检测性能：(1)表面固定的抗-IgE，然后吸附包被IgE的金纳米颗粒；(Ii)通过吸附抗-IgE包被的金纳米颗粒，在IgE特异的适配子表面生成络合物。这两种检测方案都能够直接测量毫微摩尔浓度的IgE。(C)2010爱思唯尔B V.保留所有权利
This paper describes an ultra-sensitive surface-based detection method using nanoparticle-enhanced surface plasmon resonance (SPR) for the detection of immunoglobulin E (IgE) proteins, which could potentially be used for the diagnosis of allergic diseases. Two different probes, anti-IgE and IgE specific aptamers, which can specifically interact with IgE at different epitopes were first investigated for their specific interaction with IgE using SPR. Langmuir adsorption coefficient (K-ads) values were measured as 2.0(+/- 0.22) x 10(8) M-1 and 2.2(+/- 0.20) x 10(8) M-1 for IgE interactions with anti-IgE and IgE specific aptamers. respectively. The SPR detection limit of the simple adsorption of IgE onto either anti-IgE or IgE specific aptamers was found to be about 1 nM. In order to improve the SPR detection signal for IgE, two different approaches utilizing surface formed sandwich complexes with biofunctionalized gold nanoparticles (Au-Nps) were designed and their detection performance were compared: the complexes were created via the adsorption of IgE onto (i) surface immobilized anti-IgE followed by the adsorption of IgE specific aptamer coated gold nanoparticles and (ii) IgE specific aptamer surface with the subsequent adsorption of anti-IgE coated gold nanoparticles. Both detection schemes were able to directly measure IgE at femtomolar concentrations. (C) 2010 Elsevier B V. All rights reserved