Short Pulse Laser Induces Less Inflammatory Cytokines in the Murine Retina after Laser Photocoagulation
Short Pulse Laser Induces Less Inflammatory Cytokines in the Murine Retina after Laser Photocoagulation
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DOI：
10.1159/000366520
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发表时间：
2015-01-01
影响因子：
2.1
通讯作者：
Ogura, Yuichiro
中科院分区：
文献类型：
--
作者：
Ito, Aiko;Hirano, Yoshio;Ogura, Yuichiro
目的：研究脉冲宽度对帕斯卡扫描激光光凝器和常规激光治疗后小鼠视网膜炎症细胞因子表达的影响。方法：分别用短脉冲(10ms)和常规设置(100ms)的PASCAL激光和多色激光对C57BL/6J小鼠进行视网膜激光光凝。分别于治疗前(对照组)、治疗后1d、3d、7d摘除眼球。用双抗体夹心ELISA法测定视网膜/脉络膜中炎性细胞因子(VEGF、MCP-1、RANTES和IL-6)的水平。免疫组织化学方法检测视网膜/脉络膜中血管内皮生长因子和巨噬细胞(F4/80)的表达。结果：PASCAL激光治疗组和常规激光治疗组RANTES、IL-6和MCP-1水平均显著高于对照组(P<0.05)。常规激光治疗可导致VEGF1、IL-6水平和MCP-1水平在第1天和第3天显著上调(P<；0.05)。免疫组织化学分析显示，与传统激光治疗相比，PASCAL治疗可降低VEGF和F4/80的表达水平。结论：与常规脉冲相比，短脉宽在感觉性视网膜中诱导的炎性细胞因子较少。短脉冲激光联合PASCAL可预防全视网膜光凝后黄斑水肿。(C)2015年S.Karger AG，巴塞尔
Aims:The purpose of this study was to evaluate the effect of pulse duration on the expression of inflammatory cytokines in the murine retina after laser photocoagulation treatment with a PASCAL pattern scan laser photocoagulator and conventional laser treatment. Methods: Retinal scatter laser photocoagulation was performed on C57BL/6J mice using a short pulse (10 ms) with a PASCAL laser or conventional settings (100 ms) with a multicolor laser. Eyes were enucleated before treatment (control) and 1 day, 3 days and 7 days after treatment. The levels of inflammatory cytokines (i.e., VEGF, MCP-1, RANTES and IL-6) in the retina/choroid were quantified by an ELISA. The expression patterns of VEGF and macrophages (i.e., F4/80) in the retina/choroid were evaluated by immunohistochemistry. Results: The levels of RANTES, IL-6 and MCP-1 after PASCAL and conventional laser treatments were significantly elevated compared with controls (p < 0.05). Conventional laser treatment, but not PASCAL treatment, resulted in the up-regulation of VEGF.RANTES and IL-6 levels on day 1 and MCP-1 levels on day 3 in the sensory retina were also significantly up-regulated with conventional laser treatment compared with PASCAL treatment (p < 0.05). Immunohistochemical analysis showed that PASCAL treatment was associated with lower VEGF and F4/80 expression levels compared with conventional laser treatment. Conclusions: Our data suggested that the short pulse duration induced fewer inflammatory cytokines in the sensory retina compared with the conventional pulse duration. Short pulse laser photocoagulation with the PASCAL may prevent macular edema after panretinal photocoagulation. (C) 2015 S. Karger AG, Basel