Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A
Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A
复制标题
DOI：
10.1006/dbio.1996.8463
复制
发表时间：
1997-02-01
影响因子：
2.7
通讯作者：
Moon, RT
中科院分区：
文献类型：
--
作者：
Slusarski, DC;YangSnyder, J;Moon, RT
Wnt基因编码的分泌蛋白参与受体介导的细胞-细胞信号事件，在胚胎发生中很重要，但第二信使系统的Wnfs调制尚未确定。我们报告说，异位表达的Xwnt-5A在斑马鱼胚胎中提高了频率的细胞内Ca 2+瞬变的胚盘的包膜层，而Xwnt-8没有。这些瞬变不依赖于细胞外Ca 2+。与所观察到的Ca 2+瞬变在胚胎对Xwnt-5A的反应中起作用一致，我们报告了配体激活的5-羟色胺1C型受体，其刺激PI周期活性和独立于Wnt的Ca 2+信号传导，表型模仿胚胎对Xwnt-5A的反应。这些结果表明，脊椎动物Wnt的一个子集的细胞间信号转导涉及细胞内Ca 2+信号通路的调制，这可能是由磷脂酰肌醇循环活性。(C)北京：科学出版社.
Wnt genes encode secreted proteins which are implicated in receptor-mediated cell-cell signaling events important in embryogenesis, but the second messenger systems modulated by Wnfs have not been identified. We report that ectopic expression of Xwnt-5A in zebrafish embryos enhances the frequency of intracellular Ca2+ transients in the enveloping layer of the blastodisc, whereas Xwnt-8 does not. These transients are independent of extracellular Ca2+. Consistent with the observed Ca2+ transients playing a role in responses of embryos to Xwnt-5A, We report that the ligand-activated serotonin type 1C receptor, which stimulates PI cycle activity and Ca2+ signaling independent of Wnts, phenocopies embryonic responses to Xwnt-5A. These results suggest that intercellular signaling by a subset of vertebrate Wnts involves modulation of a intracellular Ca2+ signaling pathway, which may arise from phosphatidylinositol cycle activity. (C) 1997 Academic Press.