Dual pathways in the photolysis of rhodopsin: studies using a direct chemical method.
Dual pathways in the photolysis of rhodopsin: studies using a direct chemical method.
复制标题
视紫红质光解的双重途径：使用直接化学方法的研究。
DOI：
10.1016/0042-6989(81)90183-8
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发表时间：
1981
期刊：
影响因子：
1.8
通讯作者：
Ostroy,SE
中科院分区：
文献类型：
--
作者：
Blazynski,C;Ostroy,SE
一种直接识别游离视网膜的方法已经被用来分离视紫红质光解的途径。在3°C，pH 7.0的洋地黄中，75%的牛视紫质在水解前通过Meta III465，而25%更快地降解为视网膜和视黄素。在切除的视网膜中也发现了类似的百分比。视网膜最有可能的来源是Meta II380热衰变的次要途径。牛视紫质的代谢途径依赖于pH，在pH 6.8时有一个转折点(在pH 6.5时，对视网膜的反应快42%)。这些数据还表明，Meta III465和视紫红质的消光系数大致相同。
A direct method for identifying free retinal has been used to separate the pathways in rhodopsin photolysis. At 3°C, pH 7.0, in digitonin 75% of bovine rhodopsin proceeds through Meta III465before hydrolysis, while 25% decays more rapidly to retinal plus opsin. Similar percentages are found in excised retinas (Bufo marinus). The most likely source of the retinal is a secondary pathway in Meta II380thermal decay. The pathways for bovine rhodopsin are pH dependent with a transition point at pH 6.8 (42% more rapidly to retinal at pH 6.5). These data also suggest that the extinction coefficients for Meta III465and rhodopsin are approximately the same.