Anion-exchange separation and determination of bisphosphonates and related analytes by post-column indirect fluorescence detection
Anion-exchange separation and determination of bisphosphonates and related analytes by post-column indirect fluorescence detection
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DOI：
10.1016/s0021-9673(99)00622-6
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发表时间：
1999-07-30
影响因子：
4.1
通讯作者：
Pietrzyk, DJ
中科院分区：
文献类型：
--
作者：
Lovdahl, MJ;Pietrzyk, DJ
Bisphosphonic acids and their salts can be detected after their liquid chromatographic separation by post-column indirect fluorescence detection (IFD). After separation the analyte is combined with the highly fluorescent Al3+-morin (2',3,4',5,7-pentahydroxyflavone) solution and fluorescence decreases because of the formation of the nonfluorescent Al3+-bisphosphonate complex. The decrease in fluorescence is proportional to the amount of bisphosphonate present. Separation of the multivalent anionic bisphosphonate analytes from other anions and sample matrix is achieved on a strong base anion-exchange column with a strong, basic eluent. The post-column reaction variables, which influence IFD, are identified and optimized for the detection of the bisphosphonates after separation on the anion exchanger. The method is selective, since only a few anions will undergo a reaction with the Al3+-morin solution, and sensitive, detection limit for difluoromethylene bisphosphonate, F2MDP, is 4 ng for S/N = 3. The separation-IFD method can be applied to the determination of bisphosphonates, such as F2MDP, ethane-1-hydroxy-1,1-bisphosphonic acid, dichloromethylene bisphosphonic acid, 4-amino-1-hydroxybutane-1,1-bisphosphonic acid, in biological samples. The separation-IFD method is also applicable to the detection of inositol phosphate anions. (C) 1999 Elsevier Science B.V. All rights reserved.
