Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Atrial Natriuretic Peptide Mrna Regulation by P38- Mapk in the Perfused Amphibian Heart Key Words Cell Signaling @bullet Amphibian Heart @bullet Atrial Natriuretic Peptide @bullet P38-mapk @bullet Sig
Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Atrial Natriuretic Peptide Mrna Regulation by P38- Mapk in the Perfused Amphibian Heart Key Words Cell Signaling @bullet Amphibian Heart @bullet Atrial Natriuretic Peptide @bullet P38-mapk @bullet Sig
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Biochemistr;Biochemistr And Biochemistry;Athanassios Vassilopoulos;C. Gaitanaki;P. Papazafiri;I. Beis
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作者：
Biochemistr;Biochemistr And Biochemistry;Athanassios Vassilopoulos;C. Gaitanaki;P. Papazafiri;I. Beis
心房利钠肽（ANP）是一种心脏激素，参与调节体液平衡和血压稳态。采用逆转录-聚合酶链反应扩增技术，从中国林蛙心脏组织中克隆了前心钠素原（preproANP）基因。完整cDNA的序列分析揭示了一个146个残基的肽的开放阅读框架，在C-末端携带一个17个残基的环，这是利钠肽家族的特征。在不同的刺激下，如机械，高渗或氧化应激下灌注蛙ridibula心肌诱导的转录本的快速增加，这是伴随着磷酸化p38-MAPK水平的显着增加。此外，发现1 μM SB 203580（选择性p38-MAPK抑制剂）消除了这种增加，表明p38-MAPK信号通路参与了ANP mRNA表达的调节。
Atrial natriuretic peptide (ANP) is a cardiac hormone involved in the regulation of fluid balance and blood pressure homeostasis. Using conventional reverse transcription and polymerase chain reaction and amplification, preproANP transcript from Rana ridibunda heart ventricle was cloned and characterized. Sequence analysis of the complete cDNA revealed an open reading frame for a 146-residue peptide, carrying a 17-residue ring at the C-terminus, characteristic of the natriuretic peptide family. Perfusion of Rana ridibunda myocardium under diverse stimuli such as mechanical, hyperosmotic or oxidative stress induced a rapid increase in the transcripts, which is accompanied by a significant increase in phosphorylated p38-MAPK levels. Furthermore, 1 µM SB203580, the selective p38-MAPK inhibitor, was found to abolish this increase suggesting the involvement of the p38-MAPK signalling pathway in the regulation of ANP mRNA expression.