DIRECT INVIVO MONITORING OF DOPAMINE RELEASED FROM 2 STRIATAL COMPARTMENTS IN THE RAT
DIRECT INVIVO MONITORING OF DOPAMINE RELEASED FROM 2 STRIATAL COMPARTMENTS IN THE RAT
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DOI：
10.1126/science.6857277
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
WIGHTMAN, RM
中科院分区：
文献类型：
--
作者：
EWING, AG;BIGELOW, JC;WIGHTMAN, RM
用微伏安电极监测电刺激大鼠内侧前脑束后尾状核多巴胺的释放。该技术的时间分辨率足以在秒的时间尺度上确定体内浓度变化。通过伏安法和电化学方法获得了确定释放的物质为多巴胺的直接证据。施用α-甲基-p-酪氨酸终止多巴胺的释放，尽管多巴胺的组织储存仍然存在。似乎有一个储存多巴胺的隔间，不能立即释放。该隔室似乎被氨甲酰二酸动员，因为在α-氨基甲酰二酸之后施用该药剂。甲基-p-酪氨酸使通过电刺激释放的多巴胺的浓度返回原始量的75%。
Microvoltammetric electrodes were used to monitor dopamine released in the caudate nucleus of the rat after electrical stimulation of the medial forebrain bundle. The time resolution of the technique is sufficient to determine in vivo concentration changes on a time scale of seconds. Direct evidence identifying the substance released as dopamine was obtained both with voltammetrically and pharmacologically. Administration of .alpha.-methyl-p-tyrosine terminates the release of dopamine, although tissue stores of dopamine are still present. There appears to be a compartment for dopamine storage that is not available for immediate release. This compartment appears to be mobilized by amfonelic acid, since administration of this agent after .alpha.-methyl-p-tyrosine returns the concentration of dopamine released by electrical stimulation by 75% of the original amount.