Variation in measured values of photosynthetic quantum yield in ecophysiological studies
Variation in measured values of photosynthetic quantum yield in ecophysiological studies
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DOI：
10.1007/s004420000624
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发表时间：
2001-06
期刊：
影响因子：
2.7
通讯作者：
E. Singsaas;D. Ort;E. DeLucia
中科院分区：
文献类型：
--
作者：
E. Singsaas;D. Ort;E. DeLucia
在生理生态学研究中，光合效率通常被量化为光限制的最大量子产额。四项已发表的比较研究报告说，光合效率在来源广泛的植物物种之间变化不大，量子产率接近理论上可达到的最大值。然而，许多其他已发表的研究与这一结论相矛盾，报告的量子产率低至比较研究中发现的30%。这些研究给人的印象是，某些植物，特别是生长在户外的野生植物，可能具有固有的低光合效率。为了研究这些不同解释的有效性，我们从30项已发表研究的调查中汇编了量子产率数据，并将其与两项最全面的比较量子产率研究的数据进行了比较。我们还包括我们对十个物种的量子产额观测。虽然我们的数据证实了比较研究的结果，表明最大量子产率高且不变，但文献调查数据显示量子产率值范围很广。为了研究数据收集和分析技术是否会导致低量子产率值，我们分析了光合光响应数据。在计算数据中包括超出光合光响应线性区域的数据，可能导致量子产率的大幅低估。在某些情况下，量子产率测量可以通过改变测量过程中细胞间CO2的水平来影响。我们的结论是，许多文献中报道的量子产率值的影响，一个或多个这些错误，光合作用的内在效率大多是不变的C3植物。这强调了测量和数据分析协议在获得准确可靠的量子产率数据方面的重要性。
Photosynthetic efficiency is often quantified as the light-limited, maximum quantum yield in ecophysiological studies. Four published comparative studies report that photosynthetic efficiency varies little among plant species of widely diverse origins, and that quantum yields were near the maximum theoretically attainable value. However, many other published studies contradict this conclusion, reporting quantum yields as low as 30% of those found in the comparative studies. These studies have created the impression that certain plants, particularly wild plants growing outdoors, may have intrinsically low photosynthetic efficiencies. To investigate the validity of these differing interpretations, we compiled quantum yield data from a survey of 30 published studies and compared those with data from the two most comprehensive comparative quantum yield studies. We also included quantum yield observations that we made on ten species. While our data confirm the results of the comparative studies indicating that maximum quantum yield is high and invariant, the literature survey data showed a wide range of quantum yield values. To investigate whether low quantum yield values could be caused by data collection and analysis techniques, we analyzed photosynthetic light-response data. Substantial underestimation of quantum yield could result from including in the calculation data extending beyond the linear region of the photosynthetic light response. In some cases quantum yield measurements can be influenced by changing levels of intercellular CO2during measurements. We conclude that many quantum yield values reported in the literature are affected by one or more of these errors, and the intrinsic efficiency of photosynthesis is mostly invariant among C3plants. This emphasizes the importance of the measurement and data analysis protocols in obtaining accurate and reliable quantum yield data.