SPECIFICATION OF GRADIENTS USED FOR STUDIES OF CHEMOTAXIS
SPECIFICATION OF GRADIENTS USED FOR STUDIES OF CHEMOTAXIS
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DOI：
10.1038/239517a0
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发表时间：
1972-01-01
期刊：
影响因子：
64.8
通讯作者：
BERG, HC
中科院分区：
文献类型：
--
作者：
FUTRELLE, RP;BERG, HC
19世纪80年代，费弗（Pfeffer）研究了能动细菌对各种化学物质的反应，他将含有引诱剂溶液的毛细管插入细菌悬浮液中，注意到细菌首先聚集在毛细管口附近，然后聚集在毛细管内部。Adler 2，3进一步开发了该测定法，他测量了在固定时间内进入毛细管的细菌数量。随着跟踪显微镜4的出现，可以详细分析单个细菌的反应5。为了使分析有意义，必须知道空间和时间中每个点的引诱剂浓度。因此，我们已经解决了从一个小管扩散到一个大池塘的问题。该解决方案还使我们能够解释普费弗试验的一般特征。
THE responses of motile bacteria to various chemicals were studied in the 1880s by Pfeffer1, who inserted a capillary tube containing a solution of an attractant into a suspension of bacteria and noted that the bacteria accumulated first near the mouth of the capillary and then inside. The assay has been developed further by Adler2,3, who measured the number of bacteria which enter the capillary in a fixed period of time. With the advent of the tracking microscope4it has been possible to analyse responses of individual bacteria in detail5. For the analysis to be meaningful the concentration of the attractant must be known at each point in space and time. Therefore, we have solved the problem of diffusion from a small tube into a large pond. The solution also enables us to explain the general features of the Pfeffer assay.