Limiting dilution analysis of Epstein-Barr virus-induced immunoglobulin production by human B cells
Limiting dilution analysis of Epstein-Barr virus-induced immunoglobulin production by human B cells
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EB 病毒诱导人 B 细胞产生免疫球蛋白的有限稀释分析
DOI：
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发表时间：
1983
影响因子：
15.3
通讯作者：
L. Nelson
中科院分区：
文献类型：
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作者：
R. Yarchoan;G. Tosato;R. Blaese;R. Simon;Ann David;L. Nelson
EB病毒（EBV）是一种疱疹病毒，其具有感染人B细胞并诱导它们分泌免疫球蛋白（IG）的能力。在目前的实验中，有限稀释培养物的泊松分析已用于研究EBV的B 95 -8株对人外周B细胞的活化。在所使用的培养条件下，0.2-1%的外周血B细胞被EBV激活以分泌IgM或IgG。此外，当检测含有有限数量B细胞的多个重复培养物的IgM和IgG产生时，IgM和IgG的前体独立分离;因此，单个B细胞前体成熟为分泌IgM或IgG的细胞，但不是两种类型的IG。进行使用EBV的有限稀释的额外实验以研究B细胞活化的病毒要求。这些研究表明，EBV激活B细胞产生IG与“一击”模型一致，与“两击”模型不一致。总之，这些结果表明，一种EBV病毒粒子的感染足以诱导前体外周血B细胞分泌IG，然后仅分泌一种同种型的IG。
The Epstein-Barr virus (EBV) is a herpes virus that has the capacity to infect human B cells and to induce them to secrete immunoglobulin (Ig). In the current experiments, Poisson analysis of limiting dilution cultures has been used to study the activation of human peripheral B cells by the B95-8 strain of EBV. Under the culture conditions used, 0.2-1% of peripheral blood B cells were activated by EBV to secrete IgM or IgG. In addition, when multiple replicate cultures containing limited numbers of B cells were tested for IgM and for IgG production, the precursors for IgM and IgG segregated independently; thus, individual B cell precursors matured into cells secreting IgM or IgG but not both classes of Ig. Additional experiments using limiting dilutions of EBV were undertaken to study the viral requirements for B cell activation. These studies indicated that B cell activation by EBV to produce Ig was consistent with a "one-hit" model and inconsistent with a "two-hit" model. Taken together, these results indicate that infection by one EBV virion is sufficient to induce a precursor peripheral blood B cell to secrete Ig and that only one isotype of Ig is then secreted.
根据表面同种型分离的 B 淋巴细胞表现出免疫球蛋白类别的承诺。
DOI：
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发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Teale,JM;Lafrenz,D;Klinman,NR;Strober,S
通讯作者：
Strober,S
Epstein Barr 病毒克隆转化的人 B 淋巴细胞表达免疫球蛋白。
DOI：
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发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Brown,NA;Miller,G
通讯作者：
Miller,G