Through the smoke: Use of in vivo and in vitro cigarette smoking models to elucidate its effect on female fertility
Through the smoke: Use of in vivo and in vitro cigarette smoking models to elucidate its effect on female fertility
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DOI：
10.1016/j.taap.2014.10.010
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发表时间：
2014-12-15
影响因子：
3.8
通讯作者：
Holt, Janet E.
中科院分区：
文献类型：
--
作者：
Camlin, Nicole J.;McLaughlin, Eileen A.;Holt, Janet E.
在哺乳动物出生前，卵巢内就有一定数量的卵母细胞，形成宝贵的卵巢储备。因此，香烟烟雾及其成分等环境因素对这一有限的配子库的损害对女性的生殖能力构成了重大风险。尽管迄今为止的人类研究证据表明吸烟对女性生育能力有不利影响，但这些回顾性研究是有限的，并且提出了相互矛盾的结果。为了更清楚地了解香烟烟雾及其化学成分对女性生育力的影响，已经开发了各种体内和体外动物模型。本文对4种实验模型进行了系统的综述：1）卵巢细胞和卵泡体外直接暴露于吸烟成分，2）整个卵巢组织体外直接暴露于吸烟成分，3）动物全身暴露于吸烟成分，4）动物全身暴露于香烟烟雾。我们总结了关键的发现，并强调了每个模型系统的优点和缺点，并将这些与吸烟诱导的生育能力变化中确定的分子机制联系起来。(C)2014 Elsevier Inc. All rights reserved.
A finite number of oocytes are established within the mammalian ovary prior to birth to form a precious ovarian reserve. Damage to this limited pool of gametes by environmental factors such as cigarette smoke and its constituents therefore represents a significant risk to a woman's reproductive capacity. Although evidence from human studies to date implicates a detrimental effect of cigarette smoking on female fertility, these retrospective studies are limited and present conflicting results. In an effort to more clearly understand the effect of cigarette smoke, and its chemical constituents, on female fertility, a variety of in vivo and in vitro animal models have been developed. This article represents a systematic review of the literature regarding four of experimental model types: 1) direct exposure of ovarian cells and follicles to smoking constituents' in vitro, 2) direct exposure of whole ovarian tissue with smoking constituents in vitro, 3) whole body exposure of animals to smoking constituents and 4) whole body exposure of animals to cigarette smoke. We summarise key findings and highlight the strengths and weaknesses of each model system, and link these to the molecular mechanisms identified in smoke-induced fertility changes. (C) 2014 Elsevier Inc. All rights reserved.