Interactions between fungal cellulases and films of nanofibrillar cellulose determined by a quartz crystal microbalance with dissipation monitoring (QCM-D)
Interactions between fungal cellulases and films of nanofibrillar cellulose determined by a quartz crystal microbalance with dissipation monitoring (QCM-D)
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通过具有耗散监测功能的石英晶体微天平测定真菌纤维素酶和纳米原纤纤维素膜之间的相互作用 (QCM-D)
DOI：
10.1007/s10570-017-1234-9
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发表时间：
2017-03
期刊：
影响因子：
5.7
通讯作者：
o J. Rojas
中科院分区：
文献类型：
--
作者：
Junlong Song;Fang Yang;Yu Zhang;Fanghui Hu;Shfang Wu;Yongcan Jin;Jiaqi Guo;Orl;o J. Rojas
了解酶与底物之间的相互作用以及底物在此过程中的性质变化，对于有效利用木质纤维生物质生产燃料和化学品至关重要。本文采用石英晶体耗散微天平(QCM-D)技术，研究了里氏木霉、绿色木霉、曲霉等4种真菌纤维素酶的吸附和水解行为。和黑曲霉)在纳米纤维纤维(NFC)薄膜的衬底上。用原子力显微镜对酶处理前后的纤维素膜进行了表征。结果表明，四种纤维素酶的作用方式截然不同。这些纤维素酶来自于。里森和T。病毒吸附在NFC膜上，然后进行水解，不同的色调所代表的趋势是相似和一致的。曲霉产生的纤维素酶由于频率变化很大，耗散变化不大，所以吸附在底物上形成了相当致密的一层。从纤维素酶的角度来看。尼日尔，其频率和消散的语调表现出不同的行为。利用QCM-D技术提供的多重泛音数据对除曲霉以外的纤维素酶进行粘弹性建模后，提取NFC膜和附着层的膜性质，有助于了解纤维素酶与底物之间的相互作用。
Understanding the interactions between enzymes and substrates and the property changes of the substrates during the process is vital for efficiently producing fuels and chemicals from lignocellulosic biomass. In this manuscript, quartz crystal microbalance with dissipation (QCM-D) technique was employed as a tool to investigate the adsorption and hydrolysis behaviors of four fungal cellulases (Trichoderma reesei,Trichoderma viride,Aspergillus sp. andAspergillus niger) on the substrate of nanofibrillar cellulose (NFC) film. The characterization of the cellulose films before and after enzymatic treatment was represented by atomic force microscopy. The results showed that four cellulases behaved quite differently. The cellulases fromT. reeseiandT. virideadsorbed onto NFC films and then the hydrolysis was carried out; and their trends represented by different overtones were similar and consistent. The cellulase fromAspergillus sp.adsorbed on the substrate to form a quite compact layer since substantial frequency changed with little dissipation variation. In term of the cellulase fromA. niger,its frequency and dissipation overtones exhibited diverged behaviors. After viscoelastic modeling for cellulases except forAspergillus spusing the multi-overtone data provided by QCM-D technique, the film properties of NFC film and adlayer were extracted and they could help to understand the interactions between cellulases and substrates.
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影响因子：
2.5
作者：
G. Chinga-Carrasco;K. Syverud
通讯作者：
G. Chinga-Carrasco;K. Syverud
DOI：
10.1021/jp909047t
发表时间：
2010-01
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Junlong Song;T. Yamagushi;D. J. Silva;M. Hubbe;O. Rojas
通讯作者：
Junlong Song;T. Yamagushi;D. J. Silva;M. Hubbe;O. Rojas
DOI：
--
发表时间：
1959
期刊：
--
影响因子：
--
作者：
G. Sauerbrey;G. Sauerbrey
通讯作者：
G. Sauerbrey;G. Sauerbrey
影响因子：
3.9
作者：
Cheng, Gang;Datta, Supratim;Kent, Michael S.
通讯作者：
Kent, Michael S.
影响因子：
4.8
作者：
Dan, S;Marton, I;Shoseyov, O
通讯作者：
Shoseyov, O