Coexpression Network Analysis Based Characterisation of the R2R3-MYB Family Genes in Tolerant Poplar Infected with Melampsora larici-populina
Coexpression Network Analysis Based Characterisation of the R2R3-MYB Family Genes in Tolerant Poplar Infected with Melampsora larici-populina
复制标题
基于共表达网络分析的落叶松杨树耐性杨树 R2R3-MYB 家族基因的表征
DOI：
10.3390/f13081255
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发表时间：
2022-08-01
期刊：
影响因子：
2.9
通讯作者：
Li, Danlei
中科院分区：
文献类型：
--
作者：
Chen, Qiaoli;Wang, Feng;Li, Danlei
R2 R3-MYB蛋白是植物中最丰富的一类MYB转录因子。为了研究R2 R3-MYB家族基因在杨树-E4互作中的作用，研究了两个落叶松-杨栅锈菌抗性杨树E4小种和一个不抗性白杨E4小种的转录谱。本研究共鉴定了217个R2 R3-MYB基因，通过加权基因共表达网络分析将83个R2 R3-MYB基因归属于22个不同的共表达模块。在E4侵染的早期，无论是抗性杨树还是非抗性杨树，大多数R2 R3-MYB基因没有发生变化。而在感染后2和4 d，抗病杨树的R2 R3-MYB基因表达量明显增加，表明抗病杨树R2 R3-MYB基因的差异表达可能在白杨中起重要作用。结果表明，34个R2 R3-MYB基因在感染后2和4 d表现出差异表达。其中，16个差异表达的R2 R3-MYB基因与43个防御相关基因相关，这些基因在耐受性和不耐受性杨树之间存在显着差异。在杨树与E4互作过程中，R2 R3-MYBs与其它转录因子可能存在协同调节关系。部分差异表达的R2 R3-MYB基因与类黄酮合成、IAA或游离SA信号转导相关，可能有助于启动杨树-E4互作中的防御反应。MYB 194可能是IAA和SA信号转导途径的一个重要节点。
R2R3-MYB protein is the most abundant class of MYB transcription factor family in plants. The transcript profiles of two E4 races of Melampsora larici-populina-tolerant poplars and an intolerant poplar were investigated to characterise the role of the R2R3-MYB family genes in the poplar-E4 interaction. In this study, 217 R2R3-MYBs were identified, and 83 R2R3-MYB genes were assigned to 22 different coexpression modules by weighted gene coexpression network analysis. Most R2R3-MYB genes were unchanged in the early period of E4 infection in both tolerant and intolerant poplars. However, there were obvious increases in differentially expressed R2R3-MYB genes in tolerant poplars at 2 and 4 dpi when defence responses occurred, suggesting that differently expressed R2R3-MYB genes at these time points may play an important role in poplar resistance to E4 infection. In total, 34 R2R3-MYB genes showed differential expression at 2 and 4 dpi between tolerant and intolerant poplars. Among them, 16 differentially expressed R2R3-MYB genes were related to 43 defence-related genes that had significant differences between tolerant and intolerant poplars. There might be coregulatory relationships between R2R3-MYBs and other TFs during poplar-E4 interaction. Some differentially expressed R2R3-MYB genes were related to genes involved in flavonoid biosynthesis and IAA or free SA signal transduction and might help activate defence response during poplar-E4 interaction. MYB194 could be an important node in the convergence of IAA and SA signalling.