Expression of laminin receptor in normal and carcinomatous human tissues as defined by a monoclonal antibody.
Expression of laminin receptor in normal and carcinomatous human tissues as defined by a monoclonal antibody.
复制标题
单克隆抗体定义的正常和癌性人体组织中层粘连蛋白受体的表达。
作者：
P. Hand;A. Thor;J. Schlom;C. N. Rao;L. Liotta
据推测，上皮细胞和内皮细胞通过细胞表面层粘连蛋白受体分子与基底膜的层粘连蛋白组分相互作用。也有人提出，该分子的表达可能参与了癌细胞从其起源组织的侵袭以及随后穿透血管基底膜的过程。我们在此报告使用单克隆抗体LR-3来确定层粘连蛋白受体在正常、发育不良和癌变人体组织中的表达。免疫印迹显示单克隆抗体LR-3可识别Mr 67,000层粘连蛋白受体蛋白，与癌细胞结合，约占细胞总蛋白的0.1%。许多正常的人上皮细胞和内皮细胞类型以及肺巨噬细胞都不同程度地表达层粘连蛋白受体。选定的人类乳腺癌和结肠癌比正常或发育不良的对应物结合更多的单克隆抗体LR-3。一种针对层粘连蛋白受体的单克隆抗体使得研究层粘连蛋白受体在肿瘤细胞转移和各种正常人上皮和内皮细胞类型的分化和功能中的作用成为可能。
It has been hypothesized that epithelial and endothelial cells interact with the laminin component of basement membranes via a cell surface laminin receptor molecule. It has also been proposed that the expression of this molecule may be involved in the invasion of carcinoma cells from their tissue of origin and their subsequent penetration through blood vessel basement membranes. We report here the use of a monoclonal antibody, LR-3, to define the expression of laminin receptor in normal, dysplastic, and carcinomatous human tissues. Monoclonal antibody LR-3 is shown by immunoblotting to recognize the Mr 67,000 laminin receptor protein, to bind to the carcinoma cells, and to constitute approximately 0.1% of total cellular protein. Numerous normal human epithelial and endothelial cell types, as well as pulmonary macrophages, are shown to express laminin receptor to varying degrees. Selected human mammary carcinomas and colon carcinomas are shown to bind more monoclonal antibody LR-3 than normal or dysplastic counterparts. A monoclonal antibody to laminin receptor now makes possible the study of the role of laminin receptor in tumor cell metastases and in the differentiation and function of various normal human epithelial and endothelial cell types.