Microchip electrophoretic immunoassay for serum cortisol
Microchip electrophoretic immunoassay for serum cortisol
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DOI：
10.1021/ac9508311
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发表时间：
1996-01-01
影响因子：
7.4
通讯作者：
Fuchs, M
中科院分区：
文献类型：
--
作者：
Koutny, LB;Schmalzing, D;Fuchs, M
描述了一种使用微芯片电泳系统进行的免疫测定。在竞争性测定中，游离和结合的标记抗原的分离和定量在微加工到熔融石英基底中的通道中进行。这样的微芯片是有吸引力的，因为它们的小尺寸，坚固耐用，并适应自动化处理。该测定法实现了在临床感兴趣的范围（1-60 μ g/dL）内血清中皮质醇的测定，而不需要提取或其它样品制备步骤。分离在小于30 s内进行。通过在多个通道中并行操作测定，可以实现非常高的通量。这些特性使得微芯片电泳系统成为用于临床样品快速分析的有前途的技术。
An immunoassay performed using a microchip electrophoretic system is described, Separation and quantitation of free and bound labeled antigen in a competitive assay are carried out in channels micromachined into fused silica substrates. Such microchips are attractive because of their small size, ruggedness, and amenability to automated handling. The assay achieves the determination of cortisol in blood serum over the range of clinical interest (1-60 mu g/dL) without the need for extraction or other sample preparation steps. The separation is performed in less than 30 s. Very high throughput is possible by operating the assay in multiple channels in parallel, These characteristics make microchip electrophoretic systems a promising technology for the rapid analysis of clinical samples.