The long non-coding RNA NEAT1 regulates epithelial to mesenchymal transition and radioresistance in through miR-204/ZEB1 axis in nasopharyngeal carcinoma
The long non-coding RNA NEAT1 regulates epithelial to mesenchymal transition and radioresistance in through miR-204/ZEB1 axis in nasopharyngeal carcinoma
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DOI：
10.1007/s13277-015-4773-4
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发表时间：
2016-09-01
期刊：
影响因子：
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通讯作者：
Liao, Rongwei
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文献类型：
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作者：
Lu, Yaoyong;Li, Tao;Liao, Rongwei
长链非编码 RNA (lncRNA) 在癌症进展中发挥着关键作用，包括鼻咽癌 (NPC)。然而，lncRNA是否调节鼻咽癌细胞的上皮间质转化（EMT）和放射抗性仍知之甚少。我们发现lncRNA NEAT1在NPC细胞系和组织中显着上调。 NEAT1 的敲低可以使 NPC 细胞对体外辐射敏感。进一步研究发现NEAT1通过调节EMT表型来调节放射抗性。此外，我们发现 NEAT1 和 miR-204 之间存在相互抑制。 ZEB1 被确定为 miR-204 的下游靶标，NEAT1 通过负调节 miR-204 表达上调 ZEB1 表达。综上所述，我们提出 NEAT1 通过调节 NPC 中的 miR-204/ZEB1 轴来调节 EMT 表型和放射抗性。
Long non-coding RNAs (lncRNAs) play a critical role in cancer progression, including in nasopharyngeal carcinoma (NPC). However, it is still poorly understood whether lncRNA regulates epithelial to mesenchymal transition (EMT) and radioresistance of NPC cells. We found that lncRNA NEAT1 was significantly upregulated in NPC cell lines and tissues. Knockdown of NEAT1 could sensitize NPC cells to radiation in vitro. Further investigation found that NEAT1 regulated radioresistance by modulating EMT phenotype. Furthermore, we found that there was reciprocal repression between NEAT1 and miR-204. ZEB1 was identified as a downstream target of miR-204 and NEAT1 upregulated ZEB1 expression by negatively regulating miR-204 expression. Taking together, we proposed that NEAT1 regulated EMT phenotype and radioresistance by modulating the miR-204/ZEB1 axis in NPC.