Telomere length, telomerase activity, and expressions of human telomerase mRNA component (hTERC) and human telomerase reverse transcriptase (hTERT) mRNA in pulmonary neuroendocrine tumors.
Telomere length, telomerase activity, and expressions of human telomerase mRNA component (hTERC) and human telomerase reverse transcriptase (hTERT) mRNA in pulmonary neuroendocrine tumors.
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DOI：
10.1093/jjco/hyl118
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发表时间：
2007
影响因子：
2.4
通讯作者：
Yuko Nishio;K. Nakanishi;Y. Ozeki;Shi‐Xu Jiang;T. Kameya;A. Hebisawa;M. Mukai;W. Travis;T. Franks;T. Kawai
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文献类型：
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作者：
Yuko Nishio;K. Nakanishi;Y. Ozeki;Shi‐Xu Jiang;T. Kameya;A. Hebisawa;M. Mukai;W. Travis;T. Franks;T. Kawai
背景技术端粒对于染色体结构和功能很重要，可以保护它们免遭降解。然而，很少有研究检查肺神经内分泌（NE）肿瘤中的端粒。方法 我们研究了从 70 个原发性 NE 肺肿瘤 [34 个典型类癌 (TC)、10 个非典型类癌 (AC)、16 个大细胞神经内分泌癌 (LCNEC) 和 10 个小细胞肺癌 (SCLC)] 获得的脱蜡切片。结果 TCs 中人端粒酶 mRNA 成分 (hTERC) 和 hTERT mRNA 阳性表达率分别为 58% 和 74%，ACs、LCNECs 和 SCLCs 中分别为 100% 和 100%。 LCNEC 和 SCLC 中端粒长度的改变均大于 TC。在 LCNEC 中检测到端粒酶活性，但在 TC 中未检测到。通过逆转录聚合酶链反应（RT-PCR），100%的LCNEC和TC中检测到hTERC mRNA，而67%的LCNEC中检测到hTERT mRNA，但TC中完全检测不到。结论 这些结果表明端粒长度、端粒酶活性和 hTERT mRNA 表达的改变可能 (i) 在肺神经内分泌肿瘤的发病机制中发挥作用，(ii) 成为 TC 和 LCNEC 鉴别诊断的有用工具。
BACKGROUND Telomeres are important for chromosome structure and function, protecting them against degradation. However, few studies have examined telomeres in pulmonary neuroendocrine (NE) tumors. METHODS We investigated deparaffinized sections obtained from 70 primary NE lung tumors [34 typical carcinoids (TCs), 10 atypical carcinoids (ACs), 16 large cell neuroendocrine carcinoma (LCNECs) and 10 small cell lung carcinomas (SCLCs)]. RESULTS Positive expressions of human telomerase mRNA component (hTERC) and human telomerase reverse transcriptase (hTERT) mRNA were recognized, respectively, in 58% and 74% of TCs, and in 100% and 100% of ACs, LCNECs and SCLCs. Alteration of telomere length was greater in both LCNECs and SCLCs than in TCs. Telomerase activity was detected in LCNECs, but not in TCs. By the reverse-transcriptase polymerase chain reaction (RT-PCR), hTERC mRNA was detected in 100% of LCNECs and TCs examined, while hTERT mRNA was detected in 67% of LCNECs, but not at all in TCs. CONCLUSIONS These results suggest that alterations in telomere length, telomerase activity, and the expression of hTERT mRNA may (i) play roles in pathogenesis in pulmonary neuroendocrine tumors, and (ii) be a useful tool for differential diagnosis between TCs and LCNECs.