Gold nanoparticle-based quantitative electrochemical detection of amplified human cytomegalovirus DNA using disposable microband electrodes
Gold nanoparticle-based quantitative electrochemical detection of amplified human cytomegalovirus DNA using disposable microband electrodes
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DOI：
10.1021/ac0103221
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发表时间：
2001-09-15
影响因子：
7.4
通讯作者：
Limoges, B
中科院分区：
文献类型：
--
作者：
Authier, L;Grossiord, C;Limoges, B
建立了一种灵敏定量扩增406碱基对人巨细胞病毒DNA（HCMV DNA）的电化学DNA检测方法。该测定依赖于（i）单链靶HCMV DNA与经三聚氰胺修饰的Au纳米颗粒探针的杂交，（ii）随后通过氧化金属溶解释放锚定在杂交体上的金金属原子，以及（iii）通过阳极溶出伏安法间接测定溶解的Au-III离子，该阳极溶出伏安法是在一种丝网印刷微带电极（SPMBE）上进行的。由于电沉积过程中的非线性扩散增强了Au-III的传质，SPMBE可以在小体积的静态溶液中灵敏地测定Au-III。在SPMBE处灵敏的Au-III测定与从每个金纳米颗粒探针释放的大量Au-III的组合允许检测低至5 pM的扩增的HCMV DNA片段。
An electrochemical DNA detection method has been developed for the sensitive quantification of an amplified 406-base pair human cytomegalovirus DNA sequence (HCMV DNA). The assay relies on (i) the hybridization of the single-stranded target HCMV DNA with an oligonucleotide-modified Au nanoparticle probe, (ii) followed by the release of the gold metal atoms anchored on the hybrids by oxidative metal dissolution, and (iii) the indirect determination of the solubilized Au-III ions by anodic stripping voltammetry at a sandwich-type screen-printed microband electrode (SPMBE). Due to the enhancement of the Au-III mass transfer by nonlinear diffusion during the electrodeposition time, the SPMBE allows the sensitive determination of Au-III in a small volume of quiescent solution. The combination of the sensitive Au-III determination at a SPMBE with the large number of Au-III released from each gold nanoparticle probe allows detection of as low as 5 pM amplified HCMV DNA fragment.