Rotavirus incapable of NSP6 expression can cause diarrhea in suckling mice
Rotavirus incapable of NSP6 expression can cause diarrhea in suckling mice
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DOI：
10.1099/jgv.0.001745
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发表时间：
2022-01-01
影响因子：
3.8
通讯作者：
Komoto, Satoshi
中科院分区：
文献类型：
--
作者：
Fukuda, Saori;Kugita, Masanori;Komoto, Satoshi
A组轮状病毒（RVA）基因组包含11个双链RNA，编码6个结构蛋白（VP 1-VP 4、VP 6和VP 7）和6个非结构蛋白（NSP 1-NSP 6）。在这12种轮状病毒蛋白中，NSP 6的功能研究较少。我们之前通过反向遗传学制备了源自猿猴株SA 11-L2的重组NSP 6缺陷型RVA，并发现NSP 6缺陷型病毒在细胞培养物中生长良好，尽管其生长不如亲本SA 11-L2株丰富。在这项研究中，我们检查了不能表达NSP 6的重组RVA在乳鼠中引起腹泻的效力。感染NSP 6缺陷型病毒的乳鼠明显出现腹泻，但症状较轻，腹泻持续时间短于感染真实SA 11-L2毒株的小鼠。因此，结合先前研究中对培养细胞获得的结果，可以得出结论，NSP 6不一定是体外和体内复制和致病性所必需的。
The group A rotavirus (RVA) genome comprising 11 double-stranded RNAs encodes six structural proteins (VP1-VP4, VP6, and VP7) and six non-structural proteins (NSP1-NSP6). Among these 12 rotaviral proteins, NSP6 has been less studied as to its function. We previously prepared a recombinant NSP6-deficient RVA derived from simian strain SA11-L2 by reverse genetics, and found that the NSP6-deficient virus grew well in cell culture, although its growth was less abundant than that of the parental SA11-L2 strain. In this study, we examined the potency of a recombinant RVA incapable of NSP6 expression to cause diarrhoea in suckling mice. The suckling mice infected with the NSP6-deficient virus apparently experienced diarrhoea, although the symptom was milder and the duration of diarrhoea was shorter than in the mice infected with the authentic SA11-L2 strain. Thus, together with the results obtained for cultured cells in the previous study, it can be concluded that NSP6 is not necessarily required for replication and pathogenicity in vitro and in vivo.