The relationship between glandular kallikrein and growth factor-processing proteases of mouse submaxillary gland.
The relationship between glandular kallikrein and growth factor-processing proteases of mouse submaxillary gland.
复制标题
小鼠颌下腺腺激肽释放酶与生长因子加工蛋白酶的关系。
DOI：
10.1016/s0021-9258(18)50317-3
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
E. Shooter
中科院分区：
文献类型：
--
作者：
M. Bothwell;W. H. Wilson;E. Shooter
三个高度特异性胰蛋白酶样蛋白酶从小鼠颌下腺，神经生长因子γ亚基，β神经生长因子内肽酶，表皮生长因子结合蛋白进行了测试的激肽释放酶活性。从小鼠血浆中纯化低分子量激肽原，并用作三种酶的底物，通过其诱导离体大鼠子宫收缩的能力来测定由酶释放的激肽。发现所有三种酶都具有显著的激肽原酶活性，并且最活跃的酶β神经生长因子-内肽酶具有与来自其他腺体来源的真实激肽释放酶相当的活性。基本上所有的激肽原酶活性的颌下腺共纯化β神经生长因子-内肽酶。因此，β神经生长因子-内肽酶似乎与颌下腺激肽释放酶相同。神经生长因子γ亚基、表皮生长因子结合蛋白和β神经生长因子-内肽酶具有相似的氨基酸组成和分子量，并且在免疫学上相似。比较已发表的部分一级序列数据证实了我们的结论，神经生长因子γ亚基，表皮生长因子结合蛋白，和激肽释放酶是非常密切相关的酶。据推测，这三种酶是一个更大的类似酶家族的成员，所有这些酶都参与多肽激素和生长因子前体的加工。
Three highly specific trypsin-like proteases from mouse submaxillary gland; nerve growth factor gamma subunit, beta nerve growth factor-endopeptidase, and epidermal growth factor-binding protein were tested for kallikrein activity. Low molecular weight kininogen was purified from mouse plasma and used as substrate for the three enzymes, and the kinin released by the enzymes was assayed by its ability to induce contraction of isolated rat uterus. All three enzymes were found to have significant kininogenase activity, and the most active enzyme, beta nerve growth factor-endopeptidase, has activity comparable to authentic kallikreins from other glandular sources. Essentially all of the kininogenase activity of submaxillary gland co-purifies with beta nerve growth factor-endopeptidase. Hence, beta nerve growth factor-endopeptidase appears to be identical with submaxillary gland kallikrein. Nerve growth factor gamma subunit, epidermal growth factor-binding protein, and beta nerve growth factor-endopeptidase have similar amino acid compositions and molecular weights, and are immunologically similar. Comparison of published partial primary sequence data confirms our conclusion that nerve growth factor gamma subunit, epidermal growth factor-binding protein, and kallikrein are very closely related enzymes. It is postulated that these three enzymes are members of a larger family of similar enzymes, all of which are involved in the processing of precursors to polypeptide hormones and growth factors.