PAA influence on chitosan membrane calcification
PAA influence on chitosan membrane calcification
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PAA对壳聚糖膜钙化的影响
DOI：
10.1016/j.msec.2003.08.002
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
C. Santana
中科院分区：
文献类型：
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作者：
M. Beppu;C. Santana
本文探讨了聚丙烯酸（PAA）对壳聚糖体外钙化的影响。采用扫描电镜（SEM）、X射线能谱（EDX）、X射线衍射、电位滴定和衰减全反射红外光谱（ATR-IR）研究了磷酸钙在多孔和致密（无孔）壳聚糖膜上的沉积。实验通过将壳聚糖膜在pH 7.4或7.8下在36.5 °C下在7天期间浸泡在1.0和1.5倍于人血清盐浓度的模拟体液（SBF）中来进行。SEM显微照片显示，使用这些生长溶液导致具有磷酸钙显著覆盖的基材。PAA导致更高的覆盖率，即使在较低的pH值（7.4），但沉积物的形态和化学性质是显着不同的，如果相比，纯壳聚糖膜上的沉积物。虽然PAA已经显示出促进更强烈的沉积，可能是通过提供足以成核的离子结合位点，但它不一定确定明确的晶体生长取向。
This work discusses the influence of polyacrylic acid (PAA) on chitosan in vitro calcification. Calcium phosphate deposition on porous and dense (non-porous) chitosan membranes treated or not with PAA has been studied using scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), X-ray diffraction, potentiometric titration and attenuated total reflectance infrared spectroscopy (ATR-IR). The experiments were carried out by soaking chitosan membranes in simulated body fluids (SBF), 1.0 and 1.5 times the human serum salt concentration, at 36.5 °C during 7 days at pH 7.4 or 7.8. SEM micrographs showed that the use of these growth solutions resulted in substrates with significant coverage with calcium phosphate. PAA led to a higher coverage even at lower pH (7.4), but the morphological and chemical nature of deposits was remarkably different if compared to deposits on pure chitosan membranes. Although PAA has shown to promote a more intense deposition, probably by offering ion-binding sites adequate for nucleation, it does not necessarily determine a well-defined crystal growth orientation.