Low Intensity Pulsed Ultrasound Influences the Myogenic Differentiation of Muscle Satellite Cells in a Stress Urinary Incontinence Rat Model
Low Intensity Pulsed Ultrasound Influences the Myogenic Differentiation of Muscle Satellite Cells in a Stress Urinary Incontinence Rat Model
复制标题
低强度脉冲超声影响压力性尿失禁大鼠模型中肌卫星细胞的肌源分化
DOI：
10.1016/j.urology.2018.09.020
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发表时间：
2019-01-01
期刊：
影响因子：
2.1
通讯作者：
Xin, Zhongcheng
中科院分区：
文献类型：
--
作者：
Yang, Bicheng;Li, Meng;Xin, Zhongcheng
目的 探讨低强度脉冲超声（LIPUS）对压力性尿失禁（SUI）大鼠模型的治疗效果及其对肌源性卫星细胞的影响。方法 50只Sprague-Dawley大鼠经阴道扩张和模拟产伤和绝经的双侧卵巢切除术构建SUI模型，并随机分为100 mW/cm(2) LIPUS、200 mW/cm(2) LIPUS组。 mW/cm(2) LIPUS、300 mW/cm(2) LIPUS 和未治疗对照亚组，每个亚组 10 只大鼠。十只大鼠作为模拟操作对照。 LIPUS 治疗后 1 周记录漏点压力和膀胱容量。免疫荧光染色和Western blot检测组织学变化、肌分化和信号通路。结果我们发现200 mW/cm(2) LIPUS和300 mW/cm(2) LIPUS组漏点压力和膀胱容量恢复,但100 mW/cm(2) LIPUS组没有恢复。与 SUI 未治疗组相比，200 mW/cm(2) LIPUS 组观察到更强劲的横纹肌再生。此外，我们发现LIPUS激活了肌肉卫星细胞的肌分化，这与p38磷酸化水平相关。结论LIPUS恢复了SUI大鼠模型中的渗漏点压力和膀胱容量，并激活了卫星细胞的肌分化。 (C) 2018 年由爱思唯尔公司出版
OBJECTIVE To investigate the therapeutic effect of low intensity pulsed ultrasound (LIPUS) in a stress urinary incontinence (SUI) rat model and its influence on myogenic satellite cells.METHODS Fifty Sprague-Dawley rats underwent vaginal distension and bilateral ovariectomy mimicking par- tum injury and menopause to construct SUI models, which were further randomized into 100 mW/cm(2) LIPUS, 200 mW/cm(2) LIPUS, 300 mW/cm(2) LIPUS, and none-treatment control sub-groups with 10 rats per subgroup. Ten rats served as mock operation control. Leak point pressure and bladder capacity were recorded 1 week after LIPUS treatment. Immunofluorescence staining and Western blot were performed to examine histological changes, myodifferentiation, and signaling pathway.RESULTS Here, we found the leak point pressure and bladder capacity were restored in 200 mW/cm(2) LIPUS and 300 mW/cm(2) LIPUS groups, but not in 100 mW/cm(2) LIPUS group. More robust striated muscle regeneration was observed in 200 mW/cm(2) LIPUS group comparing with the SUI none-treatment group. Moreover, we found LIPUS activated the myodifferentiation of muscle satellite cells, which is correlated to p38 phosphorylation level.CONCLUSION LIPUS restored the leak point pressure and bladder capacity, and activated satellite cell myodifferentiation in SUI rat model. (C) 2018 Published by Elsevier Inc.