Alignment-free construction of double emulsion droplet generation devices incorporating surface wettability contrast.
Alignment-free construction of double emulsion droplet generation devices incorporating surface wettability contrast.
复制标题
结合表面润湿性对比的双乳液液滴生成装置的免对准结构。
DOI：
10.1039/d3lc00584d
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发表时间：
2023
期刊：
影响因子：
6.1
通讯作者：
Aslan Y
中科院分区：
文献类型：
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作者：
Aslan Y
虽然聚二甲基硅氧烷（PDMS）是一种通用且易于使用的微流体材料，但其固有的疏水性通常需要特定的亲水处理来制造用于产生双重乳液的微芯片架构。这些额外的处理步骤经常导致复杂性增加，可能会对更广泛地使用有前途的微流体技术造成障碍。在这里，我们描述了一种无泡沫的空间亲水性PDMS图案化技术，以生产用于使用PDMS和PDMS/表面活性剂双层的组合来创建双重乳液的设备。该技术使我们能够实现选择性图案化和无污染粘合，生产可靠且可重复的水包油包水W/O/W液滴乳液。我们的方法涉及在垂直方向上处理器件，其中润湿转变对比度仅通过成像同时调节PDMS浇注速度（例如，使用移动的电话）来实现。我们成功地获得亲水性表面，没有明显的疏水性恢复使用一系列的表面活性剂浓度。液滴乳液的生产具有低变异系数，与其他更复杂的技术产生的变异系数一致（例如，内径和外径分别为3.8%和3.1%）。作为另一个实例，还证明了用于脂质体生产的方法。在未来，我们预计该技术可以应用于其他领域，包括例如试剂输送，DNA扩增和封装细胞研究。
Although polydimethylsiloxane (PDMS) is a versatile and easy-to-use material for microfluidics, its inherent hydrophobicity often necessitates specific hydrophilic treatment to fabricate microchip architectures for generating double emulsions. These additional processing steps frequently lead to increased complexity, potentially creating barriers to the wider use of promising microfluidic techniques. Here we describe an alignment-free spatial hydrophilic PDMS patterning technique to produce devices for the creation of double emulsions using combinations of PDMS and PDMS/surfactant bilayers. The technique enables us to achieve selective patterning and alignment-free bonding, producing reliable and reproducible water-in-oil-in-water W/O/W droplet emulsions. Our method involves processing devices in a vertical orientation, with the wetting transition contrast being achieved simply by imaging whilst adjusting the PDMS pouring speed (using a mobile phone, for example). We successfully obtain hydrophilic surfaces without distinguishable hydrophobic recovery using a range of surfactant concentrations. Droplet emulsions were produced with low coefficients of variation aligned with those generated with other, more complex, techniques (e.g. 3.8% and 3.1% for the inner and outer diameters, respectively). As a further example, the methods were also demonstrated for liposome production. In future we anticipate that the technique may be applied to other fields, including e.g. reagent delivery, DNA amplification, and encapsulated cell studies.