Conjugal transfer of R plasmids in Neisseria gonorrhoeae
Conjugal transfer of R plasmids in Neisseria gonorrhoeae
复制标题
淋病奈瑟氏菌中 R 质粒的接合转移
作者：
M. Roberts;S. Falkow
耐青霉素、产生β-内酰胺酶的淋病奈瑟菌菌株的出现引起了相当大的关注。我们最近发现这些淋球菌的β-内酰胺酶的产生是质粒介导的2。两个不同的R质粒已被表征；其中一株的质量为4.4 × 106，常见于远东地区军事人员及其性接触者分离的淋球菌。另一种质量为3.2 × 106的质粒是在伦敦一名妇女身上分离的菌株中发现的，我们也在利物浦的临床分离株中发现了这种质粒。DNA-DNA双工研究表明，两种淋球菌的R质粒都具有相当大一部分（约40%）的转座序列TnA，其中包括肠杆菌科、铜绿假单胞菌和流感嗜血杆菌的R质粒上常见的β-内酰胺酶基因2。
THE appearance of penicillin-resistant, β-lactamase-producing strains of Neisseria gonorrhoeae has been the cause of considerable concern1. We have recently shown that β-lactamase production by these gonococci is plasmid mediated2. Two distinct R plasmids have been characterised; one, with mass 4.4 × 106 was common in gonococci isolated from military personnel in the Far East and their sexual contacts. The other plasmid with mass 3.2 × 106 was found in a strain isolated from a woman in London3 and we have also found this plasmid species in clinical isolates from Liverpool4. DNA–DNA duplex studies have revealed that both gonococcal R plasmids posses a significant portion (about 40 %) of the transposable sequence, TnA, which includes the β-lactamase gene commonly found on R plasmids of the Enterobacteriaceae, Pseudomonas aeruginosa and Haemophilus influenzae2.