Genome Cytosine Methylation May Affect Growth and Wood Property Traits in Populations of Populus tomentosa
Genome Cytosine Methylation May Affect Growth and Wood Property Traits in Populations of Populus tomentosa
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DOI：
10.3390/f11080828
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发表时间：
2020-07
期刊：
影响因子：
2.9
通讯作者：
K. Ma;Yuepeng Song;Dong Ci;Daling Zhou;Min Tian;Deqiang Zhang
中科院分区：
文献类型：
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作者：
K. Ma;Yuepeng Song;Dong Ci;Daling Zhou;Min Tian;Deqiang Zhang
生长和木材形成是树木发育过程中至关重要且复杂的生物过程。这些生物调节过程被认为是由 DNA 甲基化控制的。然而，几乎没有直接证据表明参与木材调节的基因受到胞嘧啶甲基化的影响，从而导致表型变异。在这里，我们使用甲基化敏感扩增多态性（MSAP）方法检测表观标志物，并根据 432 个毛白杨基因型群体中的 9 个生长和木材特性性状进行表观标志物 - 性状关联分析。树高与相对全甲基化水平呈正相关，2393 个多态表观标记中有 1101 个与表型性状相关，解释了 1.1-7.8% 的表型变异。总共 116 个表观标记物被成功测序，其中 96 个序列与推定基因相关。其中，随机选取13个候选基因进行实时定量PCR（qRT-PCR）验证，结果还显示PtCYP450、PtCpn60、PtPME、PtSCP、PtGH、PtMYB、PtWRKY、PtSTP和PtABC这9个假定基因的表达与DNA甲基化水平呈负相关。因此，这表明DNA甲基化的变化可能有助于调节树木生长和木材特性。
Growth and wood formation are crucial and complex biological processes during tree development. These biological regulatory processes are presumed to be controlled by DNA methylation. However, there is little direct evidence to show that genes taking part in wood regulation are affected by cytosine methylation, resulting in phenotypic variations. Here, we detected epimarkers using a methylation-sensitive amplification polymorphism (MSAP) method and performed epimarker–trait association analysis on the basis of nine growth and wood property traits within populations of 432 genotypes of Populus tomentosa. Tree height was positively correlated with relative full-methylation level, and 1101 out of 2393 polymorphic epimarkers were associated with phenotypic traits, explaining 1.1–7.8% of the phenotypic variation. In total, 116 epimarkers were successfully sequenced, and 96 out of these sequences were linked to putative genes. Among them, 13 candidate genes were randomly selected for verification using quantitative real-time PCR (qRT-PCR), and it also showed the expression of nine putative genes of PtCYP450, PtCpn60, PtPME, PtSCP, PtGH, PtMYB, PtWRKY, PtSTP, and PtABC were negatively correlated with DNA methylation level. Therefore, it suggested that changes in DNA methylation might contribute to regulating tree growth and wood property traits.